Detection of LAMP‑2/CD107b in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human LAMP‑2/CD107b APC‑conjugated Monoclonal Antibody (Catalog # IC6228A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
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Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Lysosomal Associated Membrane Protein 2 (LAMP-2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature human LAMP-2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O-linked glycosylation (2, 3). Alternate splicing generates a human LAMP-2 isoform (LAMP-2B) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, human LAMP-2 shares approximately 64% aa sequence identity with mouse and rat LAMP-2. LAMP-2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP-2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up-regulated LAMP-2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets (9-12). LAMP-2 is a native ligand for lectins Galectin-1 and Galectin-3 (13-15).
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Lysosome-associated Membrane Glycoprotein 2
Entrez Gene IDs:
CD107 antigen-like family member B; CD107b antigen; CD107b; LAMP2; LAMP-2; LAMPB; LGP110; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2
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