Human Langerin/CD207 PerCP-conjugated Antibody

FAB2088C has been discontinued. View all Langerin/CD207 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Langerin/CD207 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 343828
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Langerin/CD207
    Tyr64-Pro328
    Accession # Q9UJ71
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Langerin/CD207 in Human Langerhans Dendritic Cells by Flow Cytometry. Human Langerhans dendritic cells (CD14+ PBMC treated with 2 ng/mL TGF- beta + 20 ng/mL rhIL-4 + 100 ng/mL rhGM-CSF for 6 days) were stained with Mouse Anti-Human Langerin/CD207 PerCP‑conjugated Mono­clonal Antibody (Catalog # FAB2088C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Langerin/CD207

Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1‑3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4‑6). Langerin also mediates endocytosis of non‑peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively.

  • References:
    1. Valladeau, J. et al. (2000) Immunity 12:71. 
    2. Valladeau, J. et al. (2003) Immunol. Res. 28:93. 
    3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317. 
    4. Stambach, N. S. and M. E. Taylor (2003) Glycobiology 13:401. 
    5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549. 
    6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51. 
    7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
    8. Hunger, R. E. et al. (2004) J. Clin. Invest. 113:701.
    9. De Witte, L. et al. (2007) Nat. Med. 13:367.
    10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
    11. Ward, E. M. et al. (2006) J. Biol. Chem. 281:15450.
    12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.
  • Entrez Gene IDs:
    50489 (Human); 246278 (Mouse)
  • Alternate Names:
    CD207 antigen; CD207 antigen, langerin; CD207 molecule, langerin; CD207; CLEC4KLangerhans cell specific c-type lectin; C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; Langerin
Related Research Areas

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