Detects human LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse LIF, recombinant human (rh) CLC, rhCNTF, rhCardiotrophin-1, rhIL-6, rhIL-11, or rhOSM is observed.
Monoclonal Mouse IgG1 Clone # 9808
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human LIF Accession # P15018
Supplied in a saline solution containing BSA and Sodium Azide.
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LIF in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human LIF PE‑conjugated Monoclonal Antibody (Catalog # IC2501P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
is a 36‑67 kDa highly glycosylated polypeptide produced by a
variety of cells including T cells, monocytes, fibroblasts,
osteoblasts and mast cells. Consistent with its many synonyms,
LIF exhibits a broad spectrum of effects on both hematopoietic and
nonhematopoietic cells. For example, LIF maintains self-renewal and pluripotency of
embryonic stem cells in mouse (but not human), up regulates the synthesis of acute phase
proteins in hepatocytes, down regulates lipoprotein lipase activity
in adipocytes, and preferentially induces a cholinergic phenotype
in sympathetic neurons. The receptor for LIF (LIF R) has been
isolated and found to be a 190 kDa type I transmembrane glycoprotein. Although this molecule binds LIF, the resultant LIF-LIF R complex
is not sufficient to transduce an intracellular signal. This capability
is provided by a 130 kDa signal transducing subunit (gp130) that is
common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin
M. Since gp130 is a ubiquitously expressed membrane protein,
the presence of LIF R (membrane-bound or soluble form) ultimately
determines the cell’s responsiveness to LIF. Cells known to express LIF R
include osteoblasts, hepatocytes, macrophages, neurons, and megakaryocytes. Human and mouse LIF exhibit 78% sequence
homology, and human LIF is biologically active on mouse cells.
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