Detects human LILRA1/CD85i/LIR-6 in direct ELISAs. In direct ELISAs, 100-400% cross-reactivity with recombinant human (rh) ILT2 is observed and no cross-reactivity rhILT3, 4, 5, 6, rhLIR-7 or -8 is observed.
Monoclonal Mouse IgG2B Clone # 586326
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human LILRA1/CD85i/LIR-6 Pro17-Asn461 Accession # O75019
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LILRA1/CD85i/LIR-6 in Human Monocytes by Flow Cytometry.
Human blood-derived monocytes were stained with Mouse Anti-Human LILRA1/CD85i/LIR-6 Monoclonal Antibody (Catalog # MAB30851, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Leukocyte Immunoglobulin-like Receptor 6 (LIR-6), also called LILRA1 or CD85i, is a glycoprotein member of the LIR family of leukocyte Ig-like receptors. As an activating LIR, LIR-6 has a short cytoplasmic tail and a charged aa within the TM domain which interacts with FcR gamma. Both four (LIR-6a) and two (LIR-6b) Ig-like domain forms are expressed by monocytes and B cells. Human LIR-6 has no non-primate ortholog, but shows up to 80% aa identity with human ILT-1c and KIR-p91/PIR-B. A mouse homolog, gp49B2, has structural similarity but limited sequence identity.
Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1
Entrez Gene IDs:
CD85 antigen-like family member I; CD85i antigen; CD85i; leukocyte immunoglobulin-like receptor subfamily A member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily A member 1; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1; LIR6; LIR-6; LIR-6Leukocyte immunoglobulin-like receptor 6; LIR6MGC126563
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