Human LILRB2/CD85d/ILT4 PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human ILT4/CD85d in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ILT1, rhILT2, rhILT3, rhILT6, rhILT7, rhILT11, rhLIR6 or rhLIR8 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 287219
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ILT4/CD85d
    Accession # ACT64556
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LILRB2/CD85d/ILT4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human LILRB2/CD85d/ILT4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2078C) or (B) Mouse IgG2A PerCP Isotype Control (Catalog # IC003C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LILRB2/CD85d/ILT4

The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) (1‑3). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC) (4). Human ILT4 is produced as a 598 amino acid (aa) precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 116 aa cytoplasmic domain. The ECD contains four Ig-like domains, and the cytoplasmic domain contains three immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5). The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64 % aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (5‑9). It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1 (7). Ligation of ILT4 induces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1 (4, 6). Activation of ILT4 inhibits signaling through Fc gamma RI (4) and Fc epsilon RI (6) and causes DC to become tolerogenic by downregulation of costimulatory molecules (10, 11). ILT4 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo (10‑12).

  • References:
    1. Suciu-Foca, N. et al. (2005) Int. Immunopharmacol. 5:7. 
    2. Hofmeister, V. and E.H. Weiss (2003) Semin. Canc. Biol. 13:317. 
    3. Hunt, J.S. et al. (2005) FASEB J. 19:681. 
    4. Finger, N.A. et al. (1998) Eur. J. Immunol. 28:3423. 
    5. Borges, L. et al. (1997) J. Immunol. 159:5192. 
    6. Colonna, M. et al. (1998) J. Immunol. 160:3096. 
    7. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. 100:8856.
    8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
    9. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
    10. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
    11. Ristich, V. et al. (2005) Eur. J. Immunol. 35:1133.
    12. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
  • Long Name:
    Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5
  • Entrez Gene IDs:
    10288 (Human)
  • Alternate Names:
    CD85 antigen-like family member D; CD85d antigen; CD85d; Ig-like transcript 4; ILT4; ILT-4; ILT4CD85d; Immunoglobulin-like transcript 4; Leukocyte immunoglobulin-like receptor 2; leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 2; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LIR2; LIR2CD85D; LIR-2subfamily A (with TM domain), member 6; member 2; MIR10; MIR10LILRA6; Monocyte/macrophage immunoglobulin-like receptor 10
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PerCP-conjugated Isotype Control

Ctrl IC003C
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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