Human Lipocalin-2/NGAL Quantikine ELISA Kit

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Lipocalin-2/NGAL ELISA Kit ELISA
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Human Lipocalin-2/NGAL Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)
0.04 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
Natural and recombinant human Lipocalin-2
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Lipocalin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lipocalin-2 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Lipocalin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lipocalin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lipocalin-2.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.14 3.45 7.54 1.05 3.37 6.92
Standard Deviation 0.041 0.107 0.334 0.083 0.204 0.387
CV% 3.6 3.1 4.4 7.9 6.1 5.6


The recovery of Lipocalin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 88-101
Urine (n=4) 90 84-97


To assess the linearity of the assay, samples containing high concentrations of Lipocalin-2 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Lipocalin-2/NGAL ELISA Linearity

Data Examples

Human Lipocalin-2/NGAL ELISA Standard Curve

Product Datasheets

Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Long Name:
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs:
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names:
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL cold Conjugate
  9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Lipocalin-2/NGAL Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Changes in brain white matter structure are associated with urine proteins in urologic chronic pelvic pain syndrome (UCPPS): A MAPP Network study
    Authors: DC Woodworth, A Dagher, A Curatolo, M Sachdev, C Ashe-McNal, BD Naliboff, JS Labus, JR Landis, JJ Kutch, EA Mayer, RS Lee, MA Moses, BM Ellingson,
    PLoS ONE, 2018;13(12):e0206807.
    Species: Human
    Sample Types: Urine
  2. Preoperative risk assessment improves biomarker detection for predicting acute kidney injury after cardiac surgery
    Authors: CC Lee, CH Chang, SW Chen, PC Fan, SW Chang, YT Chen, YY Nan, PJ Lin, FC Tsai
    PLoS ONE, 2018;13(9):e0203447.
    Species: Human
    Sample Types: Urine
  3. Prediction of delayed graft function and long-term graft survival by serum and urinary neutrophil gelatinase-associated lipocalin during the early postoperative phase after kidney transplantation
    Authors: HT Maier, MI Ashraf, C Denecke, S Weiss, F Augustin, F Messner, N Vallant, M Böcklein, C Margreiter, G Göbel, J Pratschke, D Öfner-Vela, F Aigner
    PLoS ONE, 2018;13(1):e0189932.
    Species: Human
    Sample Types: Urine
  4. Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery
    Authors: SW Ko, NH Chi, CH Wu, TM Huang, SJ Chueh, CH Wang, JH Lin, WJ Wang, JT Ting, HM Chang, R Connolly, CH Lai, LJ Tseng, VC Wu, TS Chu
    Sci Rep, 2018;8(1):1938.
    Species: Human
    Sample Types: Urine
  5. Ascorbic acid ameliorates renal injury in a murine model of contrast-induced nephropathy
    Authors: K Rollins, A Noorani, L Janeckova, T Jones, M Griffiths, MP Baker, JR Boyle
    BMC Nephrol, 2017;18(1):101.
    Species: Human
    Sample Types: Tissue Homogenates
  6. Follow Up of Value of the Intrarenal Resistivity Indices and Different Renal Biomarkers for Early Identification of Diabetic Nephropathy in Type 1 Diabetic Patients
    Authors: SAE Dayem, AEME Bohy, M Hamed, S Ahmed
    Open Access Maced J Med Sci, 2017;5(2):188-192.
    Species: Human
    Sample Types: Urine
  7. Experimental and Human Evidence for Lipocalin-2 (Neutrophil Gelatinase-Associated Lipocalin [NGAL]) in the Development of Cardiac Hypertrophy and heart failure
    Authors: FZ Marques, PR Prestes, SG Byars, SC Ritchie, P Würtz, SK Patel, SA Booth, I Rana, Y Minoda, SP Berzins, CL Curl, JR Bell, B Wai, PM Srivastava, AJ Kangas, P Soininen, S Ruohonen, M Kähönen, T Lehtimäki, E Raitoharju, A Havulinna, M Perola, O Raitakari, V Salomaa, M Ala-Korpel, J Kettunen, M McGlynn, J Kelly, ME Wlodek, PA Lewandowsk, LM Delbridge, LM Burrell, M Inouye, SB Harrap, FJ Charchar
    J Am Heart Assoc, 2017;6(6):.
    Species: Human
    Sample Types: Plasma
  8. Real-Time and Non-invasive Monitoring of the Activation of the IRE1?-XBP1 Pathway in Individuals with Hemodynamic Impairment
    Authors: B Fohlen, Q Tavernier, TM Huynh, C Caradeuc, D Le Corre, G Bertho, B Cholley, N Pallet
    EBioMedicine, 2017;0(0):.
    Species: Human
    Sample Types: Urine
  9. Neutrophil gelatinase associated lipocalin (NGAL) is elevated in type 2 diabetics with carotid artery stenosis and reduced under metformin treatment
    Authors: W Eilenberg, S Stojkovic, A Piechota-P, A Kaider, N Kozakowski, WJ Weninger, J Nanobachvi, J Wojta, I Huk, S Demyanets, C Neumayer
    Cardiovasc Diabetol, 2017;16(1):98.
    Species: Human
    Sample Types: Serum
  10. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  11. Urinary Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Patients with Obstructive Sleep Apnea
    PLoS ONE, 2016;11(5):e0154503.
    Species: Human
    Sample Types: Urine
  12. Perioperative Elevation in Cell-Free DNA Levels in Patients Undergoing Cardiac Surgery: Possible Contribution of Neutrophil Extracellular Traps to Perioperative Renal Dysfunction
    Authors: Koshi Makita
    Anesthesiol Res Pract, 2016;2016(0):2794364.
    Species: Human
    Sample Types: Serum
  13. Acute Stress Responses Are Early Molecular Events of Retinal Degeneration in Abca4-/-Rdh8-/- Mice After Light Exposure
    Authors: Tanu Parmar
    Invest Ophthalmol Vis Sci, 2016;57(7):3257-67.
    Species: Human
    Sample Types: Whole Cells
  14. Altered Systemic Adipokines in Patients with Chronic Urticaria
    Authors: Hoang Kim Tu Trinh
    Int. Arch. Allergy Immunol, 2016;171(2):102-110.
    Species: Human
    Sample Types: Serum
  15. Neonatal acute kidney injury following Valsartan exposure in utero: report of two cases
    Authors: V Drossou-Ag
    Hippokratia, 2016;20(1):73-75.
    Species: Human
    Sample Types: Urine
  16. Combined Biomarker Analysis for Risk of Acute Kidney Injury in Patients with ST-Segment Elevation Myocardial Infarction.
    Authors: Tung Y, Chang C, Chen Y, Chu P
    PLoS ONE, 2015;10(4):e0125282.
    Species: Human
    Sample Types: Serum
  17. Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis.
    Authors: Rucci N, Capulli M, Piperni S, Cappariello A, Lau P, Frings-Meuthen P, Heer M, Teti A
    J Bone Miner Res, 2015;30(2):357-68.
    Species: Human
    Sample Types: Serum
  18. Autophagy activation reduces renal tubular injury induced by urinary proteins.
    Authors: Liu, Wei Jing, Luo, Mian-Na, Tan, Jin, Chen, Wei, Huang, Lei-zhao, Yang, Chen, Pan, Qingjun, Li, Benyi, Liu, Hua-feng
    Autophagy, 2014;10(2):243-56.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Growth differentiation factor-15 (GDF-15) levels are associated with cardiac and renal injury in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass.
    Authors: Kahli A, Guenancia C, Zeller M, Grosjean S, Stamboul K, Rochette L, Girard C, Vergely C
    PLoS ONE, 2014;9(8):e105759.
    Species: Human
    Sample Types: Plasma
  20. A case-control study of pre-operative levels of serum neutrophil gelatinase-associated lipocalin and other potential inflammatory markers in colorectal cancer.
    Authors: Duvillard L, Ortega-Deballon P, Bourredjem A, Scherrer M, Mantion G, Delhorme J, Deguelte-Lardiere S, Petit J, Bonithon-Kopp C
    BMC Cancer, 2014;14(0):912.
    Species: Human
    Sample Types: Serum
  21. Unchanged plasma levels of the soluble urokinase plasminogen activator receptor in elective coronary artery bypass graft surgery patients and cardiopulmonary bypass use.
    Authors: Gozdzik W, Adamik B, Gozdzik A, Rachwalik M, Kustrzycki W, Kubler A
    PLoS ONE, 2014;9(6):e98923.
    Species: Human
    Sample Types: Plasma
  22. Urinary L-FABP and its combination with urinary NGAL in early diagnosis of acute kidney injury after cardiac surgery in adult patients.
    Biomarkers, 2013;18(1):95-101.
    Species: Human
    Sample Types: Urine
  23. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
    Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
    J Cell Sci, 2012;125(0):4475-86.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Urinary neutrophil gelatinase-associated lipocalin is a potential biomarker for renal damage in patients with systemic lupus erythematosus.
    Authors: Yang CC, Hsieh SC, Li KJ, Wu CH, Lu MC, Tsai CY, Yu CL
    J. Biomed. Biotechnol., 2012;2012(0):759313.
    Species: Human
    Sample Types: Urine
  25. Acute kidney injury biomarkers for patients in a coronary care unit: a prospective cohort study.
    Authors: Chen TH, Chang CH, Lin CY, Jenq CC, Chang MY, Tian YC, Hung CC, Fang JT, Yang CW, Wen MS, Lin FC, Chen YC
    PLoS ONE, 2012;7(2):e32328.
    Species: Human
    Sample Types: Serum
  26. Urinary neutrophil gelatinase-associated lipocalin and L-type fatty acid binding protein as diagnostic markers of early acute kidney injury after liver transplantation.
    Authors: Li Y, Zhu M, Xia Q
    Biomarkers, 2012;17(4):336-42.
    Species: Human
    Sample Types: Urine
  27. Prevalence of Chronic Kidney Disease and Progression of Disease Over Time among Patients Enrolled in the Houston West Nile Virus Cohort.
    Authors: Nolan MS, Podoll AS, Hause AM
    PLoS ONE, 2012;7(7):e40374.
    Species: Human
    Sample Types: Urine
  28. Indomethacin reduces glomerular and tubular damage markers but not renal inflammation in chronic kidney disease patients: a post-hoc analysis.
    Authors: de Borst MH, Nauta FL, Vogt L, Laverman GD, Gansevoort RT, Navis G
    PLoS ONE, 2012;7(5):e37957.
    Species: Human
    Sample Types: Urine
  29. The association between the preoperative serum levels of lipocalin-2 and matrix metalloproteinase-9 (MMP-9) and prognosis of breast cancer.
    BMC Cancer, 2012;12(0):193.
    Species: Human
    Sample Types: Serum
  30. Upregulation of lipocalin 2 in adipose tissues of severely obese women: positive relationship with proinflammatory cytokines.
    Authors: Auguet T, Quintero Y, Terra X, Martinez S, Lucas A, Pellitero S, Aguilar C, Hernandez M, Del Castillo D, Richart C
    Obesity (Silver Spring), 2011;19(12):2295-300.
    Species: Human
    Sample Types: Serum
  31. Elevated Levels of Neutrophil Gelatinase-Associated Lipocalin in Bile From Patients With Malignant Pancreatobiliary Disease.
    Authors: Zabron AA, Horneffer-van der Sluis VM, Wadsworth CA, Laird F, Gierula M, Thillainayagam AV, Vlavianos P, Westaby D, Taylor-Robinson SD, Edwards RJ, Khan SA
    Am. J. Gastroenterol., 2011;106(0):1711-7.
    Species: Human
    Sample Types: Bile
  32. Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
    Authors: Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM
    Br. J. Dermatol., 2011;165(2):302-10.
    Species: Human
    Sample Types: Complex Sample Type
  33. Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury.
    Authors: Metzger J, Kirsch T, Schiffer E, Ulger P, Mentes E, Brand K, Weissinger EM, Haubitz M, Mischak H, Herget-Rosenthal S
    Kidney Int., 2010;78(12):1252-62.
    Species: Human
    Sample Types: Urine
  34. Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin.
    Authors: Tan BK, Adya R, Shan X, Syed F, Lewandowski KC, O'Hare JP, Randeva HS
    Diabetes Care, 2009;32(1):129-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease.
    Authors: Provatopoulou X, Gounaris A, Kalogera E
    BMC Cancer, 2009;9(0):390.
    Species: Human
    Sample Types: Serum
  36. Lipocalin 2 regulation by thermal stresses: protective role of Lcn2/NGAL against cold and heat stresses.
    Authors: Roudkenar MH, Halabian R, Roushandeh AM, Nourani MR, Masroori N, Ebrahimi M, Nikogoftar M, Rouhbakhsh M, Bahmani P, Najafabadi AJ, Shokrgozar MA
    Exp. Cell Res., 2009;315(18):3140-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Lipocalin 2 promotes breast cancer progression.
    Authors: Yang J, Bielenberg DR, Rodig SJ, Doiron R, Clifton MC, Kung AL, Strong RK, Zurakowski D, Moses MA
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(10):3913-8.
    Species: Human
    Sample Types: Urine
  38. Serum concentrations of atherogenic proteins neutrophil gelatinase-associated lipocalin and its complex with matrix metalloproteinase-9 are significantly lower in women with polycystic ovary syndrome: hint of a protective mechanism?
    Authors: Diamanti-Kandarakis E, Livadas S, Kandarakis SA, Margeli A, Papassotiriou I
    Eur. J. Endocrinol., 2008;158(4):525-31.
    Species: Human
    Sample Types: Serum
  39. Retinol-binding protein 4 and lipocalin-2 in childhood and adolescent obesity: when children are not just "small adults".
    Authors: Kanaka-Gantenbein C, Margeli A, Pervanidou P, Sakka S, Mastorakos G, Chrousos GP, Papassotiriou I
    Clin. Chem., 2008;54(7):1176-82.
    Species: Human
    Sample Types: Serum


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Human Lipocalin-2/NGAL Quantikine ELISA Kit
By Anonymous on 04/24/2018
Sample Tested: Urine

Most urine samples fell within the curve at a 1 to 2 dilution. The higher NGAL samples required dilution at 1 to 10

Human Lipocalin-2/NGAL Quantikine ELISA Kit
By Anonymous on 07/18/2017
Sample Tested: Serum

Performed ideally and is probably the best kit for analyzing lipocalin2 out there! Although results should be analyzed at once after completion as saving the ELISA plate over night creates dark spots inside of the wells (see picture/data). Don´t forget to dilute serum!