Human Lipocalin-2/NGAL Quantikine ELISA Kit DLCN20: R&D Systems

Human Lipocalin-2/NGAL Quantikine ELISA Kit

(30 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)
  • Sensitivity
    0.04 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
  • Specificity
    Natural and recombinant human Lipocalin-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC115, Quantikine Immunoassay Control Set 736 for Human Lipocalin-2 - Please Inquire
Product Summary
The Quantikine Human Lipocalin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lipocalin-2 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Lipocalin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lipocalin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lipocalin-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean1.143.457.541.053.376.92
Standard Deviation0.0410.1070.3340.0830.2040.387
CV%3.63.14.47.96.15.6

Recovery

The recovery of Lipocalin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 88-101
Urine (n=4) 90 84-97
Linearity
To assess the linearity of the assay, samples containing high concentrations of Lipocalin-2 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Lipocalin-2/NGAL Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Lipocalin-2/NGAL
The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.
    • Long Name
      Neutrophil Gelatinase-associated Lipocalin
    • Entrez Gene IDs
      3934 (Human); 16819 (Mouse); 170496 (Rat);
    • Alternate Names
      24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    The conjugate must remain at 2-8 °C during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL cold Conjugate
    9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    30 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Experimental and Human Evidence for Lipocalin-2 (Neutrophil Gelatinase-Associated Lipocalin [NGAL]) in the Development of Cardiac Hypertrophy and heart failure
      Authors: FZ Marques, PR Prestes, SG Byars, SC Ritchie, P Würtz, SK Patel, SA Booth, I Rana, Y Minoda, SP Berzins, CL Curl, JR Bell, B Wai, PM Srivastava, AJ Kangas, P Soininen, S Ruohonen, M Kähönen, T Lehtimäki, E Raitoharju, A Havulinna, M Perola, O Raitakari, V Salomaa, M Ala-Korpel, J Kettunen, M McGlynn, J Kelly, ME Wlodek, PA Lewandowsk, LM Delbridge, LM Burrell, M Inouye, SB Harrap, FJ Charchar
      J Am Heart Assoc, 2017;6(6):.
      Species: Human
      Sample Type: Plasma
    2. Neutrophil gelatinase associated lipocalin (NGAL) is elevated in type 2 diabetics with carotid artery stenosis and reduced under metformin treatment
      Authors: W Eilenberg, S Stojkovic, A Piechota-P, A Kaider, N Kozakowski, WJ Weninger, J Nanobachvi, J Wojta, I Huk, S Demyanets, C Neumayer
      Cardiovasc Diabetol, 2017;16(1):98.
      Species: Human
      Sample Type: Serum
    3. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
      Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
      Cancer Res, 2017;0(0):.
      Species: Human
      Sample Type: Serum
    4. Ascorbic acid ameliorates renal injury in a murine model of contrast-induced nephropathy
      Authors: K Rollins, A Noorani, L Janeckova, T Jones, M Griffiths, MP Baker, JR Boyle
      BMC Nephrol, 2017;18(1):101.
      Species: Human
      Sample Type: Tissue Homogenates
    5. Urinary Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Patients with Obstructive Sleep Apnea
      PLoS ONE, 2016;11(5):e0154503.
      Species: Human
      Sample Type: Urine
    6. Acute Stress Responses Are Early Molecular Events of Retinal Degeneration in Abca4-/-Rdh8-/- Mice After Light Exposure
      Authors: Tanu Parmar
      Invest Ophthalmol Vis Sci, 2016;57(7):3257-67.
      Species: Human
      Sample Type: Whole Cells
    7. Combined Biomarker Analysis for Risk of Acute Kidney Injury in Patients with ST-Segment Elevation Myocardial Infarction.
      Authors: Tung Y, Chang C, Chen Y, Chu P
      PLoS ONE, 2015;10(4):e0125282.
      Species: Human
      Sample Type: Serum
    8. Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis.
      Authors: Rucci N, Capulli M, Piperni S, Cappariello A, Lau P, Frings-Meuthen P, Heer M, Teti A
      J Bone Miner Res, 2015;30(2):357-68.
      Species: Human
      Sample Type: Serum
    9. Growth differentiation factor-15 (GDF-15) levels are associated with cardiac and renal injury in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass.
      Authors: Kahli A, Guenancia C, Zeller M, Grosjean S, Stamboul K, Rochette L, Girard C, Vergely C
      PLoS ONE, 2014;9(8):e105759.
      Species: Human
      Sample Type: Plasma
    10. A case-control study of pre-operative levels of serum neutrophil gelatinase-associated lipocalin and other potential inflammatory markers in colorectal cancer.
      Authors: Duvillard L, Ortega-Deballon P, Bourredjem A, Scherrer M, Mantion G, Delhorme J, Deguelte-Lardiere S, Petit J, Bonithon-Kopp C
      BMC Cancer, 2014;14(0):912.
      Species: Human
      Sample Type: Serum
    11. Autophagy activation reduces renal tubular injury induced by urinary proteins.
      Authors: Liu, Wei Jing, Luo, Mian-Na, Tan, Jin, Chen, Wei, Huang, Lei-zhao, Yang, Chen, Pan, Qingjun, Li, Benyi, Liu, Hua-feng
      Autophagy, 2014;10(2):243-56.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Unchanged plasma levels of the soluble urokinase plasminogen activator receptor in elective coronary artery bypass graft surgery patients and cardiopulmonary bypass use.
      Authors: Gozdzik W, Adamik B, Gozdzik A, Rachwalik M, Kustrzycki W, Kubler A
      PLoS ONE, 2014;9(6):e98923.
      Species: Human
      Sample Type: Plasma
    13. Urinary L-FABP and its combination with urinary NGAL in early diagnosis of acute kidney injury after cardiac surgery in adult patients.
      Biomarkers, 2013;18(1):95-101.
      Species: Human
      Sample Type: Urine
    14. The association between the preoperative serum levels of lipocalin-2 and matrix metalloproteinase-9 (MMP-9) and prognosis of breast cancer.
      BMC Cancer, 2012;12(0):193.
      Species: Human
      Sample Type: Serum
    15. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
      Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
      J Cell Sci, 2012;125(0):4475-86.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Acute kidney injury biomarkers for patients in a coronary care unit: a prospective cohort study.
      Authors: Chen TH, Chang CH, Lin CY, Jenq CC, Chang MY, Tian YC, Hung CC, Fang JT, Yang CW, Wen MS, Lin FC, Chen YC
      PLoS ONE, 2012;7(2):e32328.
      Species: Human
      Sample Type: Serum
    17. Indomethacin reduces glomerular and tubular damage markers but not renal inflammation in chronic kidney disease patients: a post-hoc analysis.
      Authors: de Borst MH, Nauta FL, Vogt L, Laverman GD, Gansevoort RT, Navis G
      PLoS ONE, 2012;7(5):e37957.
      Species: Human
      Sample Type: Urine
    18. Prevalence of Chronic Kidney Disease and Progression of Disease Over Time among Patients Enrolled in the Houston West Nile Virus Cohort.
      Authors: Nolan MS, Podoll AS, Hause AM
      PLoS ONE, 2012;7(7):e40374.
      Species: Human
      Sample Type: Urine
    19. Urinary neutrophil gelatinase-associated lipocalin and L-type fatty acid binding protein as diagnostic markers of early acute kidney injury after liver transplantation.
      Authors: Li Y, Zhu M, Xia Q
      Biomarkers, 2012;17(4):336-42.
      Species: Human
      Sample Type: Urine
    20. Urinary neutrophil gelatinase-associated lipocalin is a potential biomarker for renal damage in patients with systemic lupus erythematosus.
      Authors: Yang CC, Hsieh SC, Li KJ, Wu CH, Lu MC, Tsai CY, Yu CL
      J. Biomed. Biotechnol., 2012;2012(0):759313.
      Species: Human
      Sample Type: Urine
    21. Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
      Authors: Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM
      Br. J. Dermatol., 2011;165(2):302-10.
      Species: Human
      Sample Type: Complex Sample Type
    22. Elevated Levels of Neutrophil Gelatinase-Associated Lipocalin in Bile From Patients With Malignant Pancreatobiliary Disease.
      Authors: Zabron AA, Horneffer-van der Sluis VM, Wadsworth CA, Laird F, Gierula M, Thillainayagam AV, Vlavianos P, Westaby D, Taylor-Robinson SD, Edwards RJ, Khan SA
      Am. J. Gastroenterol., 2011;106(0):1711-7.
      Species: Human
      Sample Type: Bile
    23. Upregulation of lipocalin 2 in adipose tissues of severely obese women: positive relationship with proinflammatory cytokines.
      Authors: Auguet T, Quintero Y, Terra X, Martinez S, Lucas A, Pellitero S, Aguilar C, Hernandez M, Del Castillo D, Richart C
      Obesity (Silver Spring), 2011;19(12):2295-300.
      Species: Human
      Sample Type: Serum
    24. Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury.
      Authors: Metzger J, Kirsch T, Schiffer E, Ulger P, Mentes E, Brand K, Weissinger EM, Haubitz M, Mischak H, Herget-Rosenthal S
      Kidney Int., 2010;78(12):1252-62.
      Species: Human
      Sample Type: Urine
    25. Lipocalin 2 promotes breast cancer progression.
      Authors: Yang J, Bielenberg DR, Rodig SJ, Doiron R, Clifton MC, Kung AL, Strong RK, Zurakowski D, Moses MA
      Proc. Natl. Acad. Sci. U.S.A., 2009;106(10):3913-8.
      Species: Human
      Sample Type: Urine
    26. Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin.
      Authors: Tan BK, Adya R, Shan X, Syed F, Lewandowski KC, O'Hare JP, Randeva HS
      Diabetes Care, 2009;32(1):129-31.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease.
      Authors: Provatopoulou X, Gounaris A, Kalogera E
      BMC Cancer, 2009;9(0):390.
      Species: Human
      Sample Type: Serum
    28. Lipocalin 2 regulation by thermal stresses: protective role of Lcn2/NGAL against cold and heat stresses.
      Authors: Roudkenar MH, Halabian R, Roushandeh AM, Nourani MR, Masroori N, Ebrahimi M, Nikogoftar M, Rouhbakhsh M, Bahmani P, Najafabadi AJ, Shokrgozar MA
      Exp. Cell Res., 2009;315(18):3140-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. Serum concentrations of atherogenic proteins neutrophil gelatinase-associated lipocalin and its complex with matrix metalloproteinase-9 are significantly lower in women with polycystic ovary syndrome: hint of a protective mechanism?
      Authors: Diamanti-Kandarakis E, Livadas S, Kandarakis SA, Margeli A, Papassotiriou I
      Eur. J. Endocrinol., 2008;158(4):525-31.
      Species: Human
      Sample Type: Serum
    30. Retinol-binding protein 4 and lipocalin-2 in childhood and adolescent obesity: when children are not just "small adults".
      Authors: Kanaka-Gantenbein C, Margeli A, Pervanidou P, Sakka S, Mastorakos G, Chrousos GP, Papassotiriou I
      Clin. Chem., 2008;54(7):1176-82.
      Species: Human
      Sample Type: Serum
    Expand to show all 30 Citations
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    Quantikine Immunoassay Control Set 736 for Human Lipocalin-2

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    : Human Lipocalin-2/NGAL Quantikine ELISA Kit [DLCN20]

    Summary

    Sample TestedSerum

    Other Experimental Details

    Other Experimental DetailsPerformed ideally and is probably the best kit for analyzing lipocalin2 out there! Although results should be analyzed at once after completion as saving the ELISA plate over night creates dark spots inside of the wells (see picture/data). Don´t forget to dilute serum!

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