Human LIR-6/CD85i APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human LIR-6 in direct ELISAs. In direct ELISAs, 100-400% cross-reactivity with recombinant human (rh) ILT2 is observed and no cross-reactivity with rhILT3, 4, 5, 6, rhLIR-7 or -8 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 586326
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LIR-6
    Accession # O75019
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LIR‑6 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human LIR‑6 APC‑conjugated Monoclonal Antibody (Catalog # FAB30851A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LILRA1/CD85i/LIR-6

Leukocyte Immunoglobulin-like Receptor 6 (LIR-6), also called LILRA1 or CD85i, is a glycoprotein member of the LIR family of leukocyte Ig-like receptors. As an activating LIR, LIR-6 has a short cytoplasmic tail and a charged amino acid (aa) within the TM domain which interacts with FcR gamma. Both four (LIR-6a) and two (LIR-6b) Ig-like domain forms are expressed by monocytes and B cells. Human LIR-6 has no non-primate ortholog, but shows up to 80% aa identity with human ILT-1c and KIR-p91/PIR-B. A mouse homolog, gp49B2, has structural similarity but limited sequence identity.

  • Long Name:
    Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1
  • Entrez Gene IDs:
    11024 (Human)
  • Alternate Names:
    CD85 antigen-like family member I; CD85i antigen; CD85i; leukocyte immunoglobulin-like receptor subfamily A member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily A member 1; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1; LIR6; LIR-6; LIR-6Leukocyte immunoglobulin-like receptor 6; LIR6MGC126563
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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