Detects human LRIG3 in direct ELISAs and Western blots. Detects mouse LRIG3 in Western blots. Mouse LRIG3 detection in Flow Cytometry was not tested. In direct ELISAs, no cross-reactivity with recombinant
human LRIG1 is observed.
Monoclonal Mouse IgG2B Clone # 814834
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human LRIG3 Asp28-Thr807 Accession # Q6UXM1
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of LRIG3 in KATO‑III Human Cell Line by Flow Cytometry.
KATO‑III human gastric carcinoma cell line was stained with Mouse Anti-Human LRIG3 APC‑conjugated Monoclonal Antibody (Catalog # FAB3495A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
LRIG3 (Leucine-rich Repeats and Ig-like domains-3) is an approximately 140 kDa type I transmembrane glycoprotein member of the mammalian LRIG glycoprotein family. This family contains three members who share 45-50% amino acid (aa) sequence identity (1). All members contain at least fifteen LRRs, accompanied by two flanking cysteine-rich regions and three C2-type Ig-like domains in their extracellular domains (ECD) (1). LRIG3 mRNA is widely expressed, with highest levels in stomach, skin, thyroid and small intestine (1). Human LRIG3 is synthesized as a 1119 amino acid (aa) precursor. It contains a 24 aa signal sequence, a 785 aa ECD, a 21 aa transmembrane sequence, and a 289 aa intracellular region. One splice variant exists that has a 19 aa substitution for the first 79 aa of the standard (or long) form. This substitution appears to encode an alternate signal sequence, resulting in a mature protein that lacks the first and part of the second LRR. LRIG1, a related family member, is known to bind the EGF family receptors ErbB1-4, via either its LRR or Ig-like domains. It also binds the ubiquitin ligase, c-Cbl, and promotes ubiquitination, internalization and destruction of these receptors (2, 3). LRIG3, by contrast, would appear to oppose LRIG1 activity, in particular by promoting the expression of ErbB1-4. LRIG1, in kind, downregulates LRIG3 activity by inducing its proteolytic degradation (4). Within the cell, LRIG3 is expressed in the perinuclear region as well as on the cell surface. Perinuclear location of LRIG3 in grade III and IV astrocytic tumors has been associated with better patient survival (5). Human LRIG3 ECD shares 91%, 92%, 95% and 98% aa sequence identity with mouse, rat, bovine and canine LRIG3 ECD, respectively.
Guo, D. et al. (2004) Genomics 84:157.
Gur, G. et al. (2004) EMBO J. 23:3270.
Laederich, M.B. (2004) J. Biol. Chem. 279:47050.
Simion, C. et al. (2014) Endocr. Relat. Cancer 21: R431.
Guo, D. et al. (2006) Acta Neuropathol. (Berl.) 111:238.
Leucine-rich Repeats and Immunoglobulin-like Domains 3
Entrez Gene IDs:
121227 (Human); 320398 (Mouse)
FLJ90440; KIAA3016; leucine-rich repeats and immunoglobulin-like domains 3; leucine-rich repeats and immunoglobulin-like domains protein 3; LIG-3; LIG3FLJ26573; LIRG3; LRIG3
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