Human LRIG3 APC-conjugated Antibody

FAB3495A has been discontinued. View all LRIG3 products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects human LRIG3 in direct ELISAs and Western blots. Detects mouse LRIG3 in Western blots. Mouse LRIG3 detection in Flow Cytometry was not tested. In direct ELISAs, no cross-reactivity with recombinant human LRIG1 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 814834
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LRIG3
    Accession # Q6UXM1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of LRIG3 in KATO‑III Human Cell Line by Flow Cytometry. KATO‑III human gastric carcinoma cell line was stained with Mouse Anti-Human LRIG3 APC‑conjugated Monoclonal Antibody (Catalog # FAB3495A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LRIG3

LRIG3 (Leucine-rich Repeats and Ig-like domains-3) is an approximately 140 kDa type I transmembrane glycoprotein member of the mammalian LRIG glycoprotein family. This family contains three members who share 45-50% amino acid (aa) sequence identity (1). All members contain at least fifteen LRRs, accompanied by two flanking cysteine-rich regions and three C2-type Ig-like domains in their extracellular domains (ECD) (1). LRIG3 mRNA is widely expressed, with highest levels in stomach, skin, thyroid and small intestine (1). Human LRIG3 is synthesized as a 1119 amino acid (aa) precursor. It contains a 24 aa signal sequence, a 785 aa ECD, a 21 aa transmembrane sequence, and a 289 aa intracellular region. One splice variant exists that has a 19 aa substitution for the first 79 aa of the standard (or long) form. This substitution appears to encode an alternate signal sequence, resulting in a mature protein that lacks the first and part of the second LRR. LRIG1, a related family member, is known to bind the EGF family receptors ErbB1-4, via either its LRR or Ig-like domains. It also binds the ubiquitin ligase, c-Cbl, and promotes ubiquitination, internalization and destruction of these receptors (2, 3). LRIG3, by contrast, would appear to oppose LRIG1 activity, in particular by promoting the expression of ErbB1-4. LRIG1, in kind, downregulates LRIG3 activity by inducing its proteolytic degradation (4). Within the cell, LRIG3 is expressed in the perinuclear region as well as on the cell surface. Perinuclear location of LRIG3 in grade III and IV astrocytic tumors has been associated with better patient survival (5). Human LRIG3 ECD shares 91%, 92%, 95% and 98% aa sequence identity with mouse, rat, bovine and canine LRIG3 ECD, respectively.

  • References:
    1. Guo, D. et al. (2004) Genomics 84:157.
    2. Gur, G. et al. (2004) EMBO J. 23:3270.
    3. Laederich, M.B. (2004) J. Biol. Chem. 279:47050.
    4. Simion, C. et al. (2014) Endocr. Relat. Cancer 21: R431.
    5. Guo, D. et al. (2006) Acta Neuropathol. (Berl.) 111:238.
  • Long Name:
    Leucine-rich Repeats and Immunoglobulin-like Domains 3
  • Entrez Gene IDs:
    121227 (Human); 320398 (Mouse)
  • Alternate Names:
    FLJ90440; KIAA3016; leucine-rich repeats and immunoglobulin-like domains 3; leucine-rich repeats and immunoglobulin-like domains protein 3; LIG-3; LIG3FLJ26573; LIRG3; LRIG3
Related Research Areas


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Human LRIG3 APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order


Customer Information
Related Products

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Panels For

Learn More
Monoclonal Antibodies
R&D Systems Guarantee