Human MESP1 Antibody

Name: Human MESP1 Antibody
Brand: R&D Systems
SKU: MAB92192
Rating: 5 (1 reviews)

Catalog #: MAB92192 1 Review Datasheet / COA / SDS

Newer Version Available: MAB92193

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Discontinued Product

MAB92192 has been discontinued and is replaced by MAB92193.

Detection of Human MESP1 by Western Blot.

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Product Details

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MESP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MESP1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 939816

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human MESP1
Met1-Gln85
Accession # Q9BRJ9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1-2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MESP1 by Western Blot. Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline for overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB92192) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. Cells provided courtesy of Michael Kyba's lab, University of Minnesota.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MESP1

Mesoderm posterior protein 1 (Mesp-1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. Mesp-1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that Mesp-1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.

Long Name

Mesoderm Posterior BHLH Transcription Factor 1

Entrez Gene IDs

55897 (Human); 17292 (Mouse); 308766 (Rat)

Alternate Names

bHLHc5; bHLHc5MGC10676; Class C basic helix-loop-helix protein 5; mesoderm posterior 1 homolog (mouse); mesoderm posterior protein 1; MESP1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Human MESP1 Antibody

Average Rating: 5 (Based on 1 Review)

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Human MESP1 Antibody

By Anonymous on 02/01/2022

Application: WB Sample Tested: Testis tissue Species: Human

Western Blot Human MESP1 Antibody MAB92192