Human MMP-16/MT3-MMP APC-conjugated Antibody

FAB1785A has been discontinued. View all MMP-16/MT3-MMP products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human MMP-16/MT3-MMP in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MMP-14, -15, or -24 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 782005
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human MMP-16/MT3-MMP
    Ala32-Pro535
    Accession # P51512
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MMP‑16/MT3‑MMP in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human MMP‑16/MT3‑MMP APC‑conjugated Monoclonal Antibody (Catalog # FAB1785A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MMP-16/MT3-MMP

Matrix Metalloproteinase 16 (MMP-16), also known as MT3-MMP, is a 62-63 kDa member of the peptidase M10A family of enzymes (1,2). MMP-16 is found on capillary endothelial cells, cortical neurons cerebellar granule cells, granulosa lutein and theca lutein cells, and melanoma cells (3-6). MMP-16 has been shown to cleave proMMP-2 in a complex with chondroitin-4 sulfate (7). It also cleaves NgR1 and select matrix proteins (4). Notably, cleavage of MMP-14 inactivates this molecules, arresting cell migration (6). Structurally, proMMP-16 is 65-66 kDa in size and consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplasmic tail (1). There is one soluble 47-48 kDa splice variant that retains proteolytic activity. Over amino acids (aa) 32-535, human and mouse MMP-16 share 98% amino acid sequence identity.

  • References:
    1. Takino, T. et al. (1995) J. Biol. Chem. 270:23013.
    2. Nagase, H. et al. (2006) Cardiovasc. Res. 69:562.
    3. Lozito, T.P. and R.S. Tuan (2012) J. Cell. Physiol. 227:534.
    4. Ferraro, G.B. et al. (2011)  J. Biol. Chem. 286:31418.
    5. Puttabyatapppa, M, et al. (2014)  Biol. Reprod. 91:1.
    6. Tatti, O. et al. (2015) Cancer Res. 75:2083.
    7. Iida, J. et al. (2007) Biochem. J. 403:533.
    8. Shimada, T. et al. (1999) Eur. J. Biochem. 262:907.
    9. Matsumodo, S., et al. (1997) Biochim. Biophys. Acta. 1354:159.
  • Long Name:
    Matrix Metalloproteinase 16/Membrane Type 3 MMP
  • Entrez Gene IDs:
    4325 (Human)
  • Alternate Names:
    chromosome 8 open reading frame 57; DKFZp761D112; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 16 (membrane-inserted); matrix metalloproteinase 16 (membrane-inserted); matrix metalloproteinase-16; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP16; MMP-16; MMPX2; MMP-X2; MT3MMP; MT3-MMP; MT3-MMPC8orf57; MT-MMP 3; MT-MMP2; MTMMP3; MT-MMP3; Putative transmembrane protein C8orf57
Related Research Areas

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