|Detection of Human and Mouse AHR by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, mouse lung tissue, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|AHR in C2C12 Mouse Cell Line. AHR was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
AHR (Aryl-hydrocarbon receptor; also bHLHE76) is a 100-105 kDa member of the bHLH/PAS transcription factor family. It is widely expressed and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a downregulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing Tap73 and Apaf1 genes. Mouse AHR precursor is 848 amino acids (aa) in length. It contains a nine aa prosegment, plus an 839 aa mature molecule that contains a DNA binding motif (aa 12-39), a bHLH region (aa 40-80), two PAS domains (aa 116-336) and one PAC segment that stabilizes the PAS domains (aa 342-383). There are multiple alleles for mouse AHR. One 95-97 kDa allele shows a premature truncation after Ser805, while a second 112 kDa allele shows a 41 aa substitution for aa 843-848. Over aa 706-805, mouse AHR shares 87% and 63% aa identity with rat and human AHR, respectively.
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