Human/Mouse Desmocollin-1 APC-conjugated Antibody

FAB7367A has been discontinued. View all Desmocollin-1 products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse Desmocollin-1 in ELISA and Western blot. In direct ELISA, 100% cross-reactivity with recombinant human Desmocollin-1 is observed, and no cross-reactivity with recombinant mouse Desmocollin-2 or -3 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 772906
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-1
    Arg135-Lys691
    Accession # P55849
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Desmocollin‑1 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse Desmocollin‑1 APC‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB7367A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Desmocollin‑1 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Rat Anti-Mouse Desmocollin‑1 APC‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB7367A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Desmocollin-1

DSC-1 (Desmocollin [Greek for "glue-that-binds"]-1) is an approximately 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including the spinous and granular layers of keratinized and nonkeratinized epithelia of the oral cavity and skin. DSC‑1 expression is induced by DSG-1. It serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, homodimeric DSC-1 may form heterotypic interactions with DSG-1 in-trans, and bind to the cytoskeleton intracellularly via plakophilin-1. Mature mouse DSC-1 is a 760 amino acid (aa) type I transmembrane glycoprotein (aa 135-691). The mature molecule contains a 557 aa extracellular region with five cadherin domains (aa 135-682), and a 172 aa cytoplasmic domain. There is one splice variant that shows an 11 aa substitution for aa 822-886. Over aa 135-691, mouse DSC‑1 shares 82% aa sequence identity with human DSC-1.

  • Entrez Gene IDs:
    1823 (Human); 13505 (Mouse); 291759 (Rat)
  • Alternate Names:
    cadherin family member 1; CDHF1; desmocollin 1; Desmocollin1; Desmocollin-1; desmosomal glycoprotein 2/3; DG2/DG3 ; DG2/DG3; DSC1
Related Research Areas

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