Detects mouse Desmocollin-1 in ELISA and Western blot. In direct ELISA, 100% cross-reactivity with
recombinant human Desmocollin-1 is observed, and no cross-reactivity with
recombinant mouse Desmocollin-2 or -3 is observed.
Monoclonal Rat IgG2A Clone # 772906
Protein A or G purified from hybridoma culture supernatant
Detection of Desmocollin‑1 in B16‑F1 Mouse Cell Line by Flow Cytometry.
B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse Desmocollin‑1 APC‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB7367A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Desmocollin‑1 in A549 Human Cell Line by Flow Cytometry.
A549 human lung carcinoma cell line was stained with Rat Anti-Mouse Desmocollin‑1 APC‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB7367A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
DSC-1 (Desmocollin [Greek for "glue-that-binds"]-1) is an approximately 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including the spinous and granular layers of keratinized and nonkeratinized epithelia of the oral cavity and skin. DSC‑1 expression is induced by DSG-1. It serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, homodimeric DSC-1 may form heterotypic interactions with DSG-1 in-trans, and bind to the cytoskeleton intracellularly via plakophilin-1. Mature mouse DSC-1 is a 760 amino acid (aa) type I transmembrane glycoprotein (aa 135-691). The mature molecule contains a 557 aa extracellular region with five cadherin domains (aa 135-682), and a 172 aa cytoplasmic domain. There is one splice variant that shows an 11 aa substitution for aa 822-886. Over aa 135-691, mouse DSC‑1 shares 82% aa sequence identity with human DSC-1.
Entrez Gene IDs:
1823 (Human); 13505 (Mouse); 291759 (Rat)
cadherin family member 1; CDHF1; desmocollin 1; Desmocollin1; Desmocollin-1; desmosomal glycoprotein 2/3; DG2/DG3 ; DG2/DG3; DSC1
Have you used Human/Mouse Desmocollin-1 APC-conjugated Antibody?
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