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Human/Mouse FoxP3 APC-conjugated Antibody

  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Flow Cytometry: FoxP3 Antibody [Allophycocyanin] [FPK8214A-025] - Detection of FoxP3 in Human PBMC Natural Regulatory T Cells (Tregs) by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) natural Tregs were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (R&D Systems, Catalog # FAB3791F) and (B) Mouse Anti-Human CD25/ IL2-R alpha PE-conjugated Monoclonal Antibody (R&D Systems, Catalog # FAB1020P), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC-conjugated Antigen Affinity-purified Monoclonal Antibody (R&D Systems, Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (R&D Systems, Catalog # FC012). Cells were gated on lymphocytes.
Preparation and Storage
  • Stability & Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. Protect from light.
Background: Forkhead Box P3
Human FOXP3 is a 47 kDa member of the P subclass of the forkhead box family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (forkhead) DNA-binding domain. In human, FOXP3 is found in CD4+, CD8+ and CD4+CD25+ T cells and directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells.
  • Long Name:
    Forkhead Box P3
  • Entrez Gene IDs:
    50943 (Human); 20371 (Mouse);
  • Alternate Names:
    AIIDMGC141961; forkhead box P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; JM2; PIDXMGC141963; Scurfin; SCURFIN; scurfin; XPIDpolyendocrinopathy, enteropathy, X-linked
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may click here to view all FAQs for Primary Antibodies

Order Details

  • Catalog #
    FPK8214A

Contact Information




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