Human/Mouse Frizzled-1 Fluorescein-conjugated Antibody

    
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  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Frizzled-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rm Frizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 162531
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
    Gln1-His248 (Met122Ile)
    Accession # O70421
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Frizzled‑1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled‑1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB11201F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Frizzled‑1 in D3 Mouse Cell Line treated with Retinoic Acid by Flow Cytometry. D3 mouse embryonic stem cell line treated with retinoic acid was stained with Rat Anti-Human/Mouse Frizzled‑1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB11201F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Frizzled-1
Members of the Fz/Smo family of proteins serve as receptors in the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled-1 (Fzd-1) is a 75-80 kDa 7-transmembrane glycoprotein found on chondrocytes and in the presynaptic membranes of neurons. It is also reported to be found on fibroblasts, osteoblasts, enothelium and vascular smooth muscle, and in the embryo in mesoderm. Fzd-1 interacts with LRP-5 to generate a receptor complex for Wnts. Wnts known to bind Fzd-1 include Wnt-1, -2, -3, -3a, and -8. Over amino acids (aa) 1-248, human and mouse Fzd-1 share 87% aa sequence identity.
  • Entrez Gene IDs:
    8321 (Human); 14362 (Mouse)
  • Alternate Names:
    DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Fluorescein-conjugated Antibody

Ctrl IC006F 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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