Detects human and mouse Frizzled-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rm Frizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed.
Monoclonal Rat IgG2A Clone # 162531
Protein A or G purified from hybridoma culture supernatant
Detection of Frizzled‑1 in HEK293 Human Cell Line by Flow Cytometry.
HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled‑1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB11201F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Frizzled‑1 in D3 Mouse Cell Line treated with Retinoic Acid by Flow Cytometry.
D3 mouse embryonic stem cell line treated with retinoic acid was stained with Rat Anti-Human/Mouse Frizzled‑1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB11201F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Members of the Fz/Smo family of proteins serve as receptors in the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled-1 (Fzd-1) is a 75-80 kDa 7-transmembrane glycoprotein found on chondrocytes and in the presynaptic membranes of neurons. It is also reported to be found on fibroblasts, osteoblasts, enothelium and vascular smooth muscle, and in the embryo in mesoderm. Fzd-1 interacts with LRP-5 to generate a receptor complex for Wnts. Wnts known to bind Fzd-1 include Wnt-1, -2, -3, -3a, and -8. Over amino acids (aa) 1-248, human and mouse Fzd-1 share 87% aa sequence identity.
Have you used Human/Mouse Frizzled-1 Fluorescein-conjugated Antibody?
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