Human/Mouse Frizzled-3 PE-conjugated Antibody

FAB1001P has been discontinued. View all Frizzled-3 products.
    
Datasheet / CoA / SDS
Product Details
FAQs
Reviews
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse Frizzled-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -4, -7, or -8 is observed. By flow cytometry and immunohistochemistry, this antibody also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. This antibody also detects Frizzled-3 on human HEK293 cells by immunocytochemistry and flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 169310
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
    Met1-Val157
    Accession # Q61086
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Frizzled‑3 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB1001P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Frizzled‑3 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Human/Mouse Frizzled‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB1001P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Frizzled-3
Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.
  • Entrez Gene IDs:
    7976 (Human); 14365 (Mouse)
  • Alternate Names:
    frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Human/Mouse Frizzled-3 PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
Associated Pathways
R&D Systems Guarantee