Human/Mouse Frizzled-4 PE-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3,
    -6, -7, -8, or recombinant human Frizzled-5 is observed. Detects Frizzled-4 by flow cytometry and immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3.
  • Source
    Monoclonal Rat IgG2A Clone # 145901
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
    Phe37-Glu180
    Accession # Q61088
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Frizzled‑4 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled‑4 PE‑conjugated Monoclonal Antibody (Catalog # FAB194P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Frizzled‑4 in RA Treated D3 Mouse Cell Line by Flow Cytometry. RA treated D3 mouse embryonic stem cell line was stained with Rat Anti-Human/Mouse Frizzled‑4 PE‑conjugated Monoclonal Antibody (Catalog # FAB194P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Frizzled-4
Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein member of the Frizzled family that belongs to the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 3). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120  amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 186 aa N-terminal extracellular region, and a 38 aa C-terminal cytoplasmic domain (4). The aa sequence expressed here spans aa 37-180, and shares 93%, 94%, 90%, 89% and 88% aa sequence identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (3). Frizzled-4 is unusual in binding a non-Wnt ligand, Norrin, in addition to binding Wnt ligands (1, 5, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (5, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 5, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 5, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).
  • References:
    1. Huang, H-C. and P.S. Klein (2004) Genome Biol. 5:234.
    2. Parmalee, N.L. and J. Kitajewski (2008) Curr. Drug Targets 9:558.
    3. Sagara, N. et al. (2001) Biochem. Biophys. Res. Commun. 282:750.
    4. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
    5. Xu, Q. et al. (2004) Cell 116:883.
    6. Smallwood, P.M. et al. (2007) J. Biol. Chem. 282:4057.
    7. Wang, Y. et al. (2001) J. Neurosci. 21:4761.
    8. Robitaille, J. et al. (2002) Nat. Genet. 32:326.
    9. Hsieh, M. et al. (2005) Biol. Reprod. 73:1135.
  • Entrez Gene IDs:
    8322 (Human); 14366 (Mouse)
  • Alternate Names:
    CD344 antigen; CD344; EVR1; exudative vitreoretinopathy 1; FEVR; frizzled (Drosophila) homolog 4; frizzled homolog 4 (Drosophila); Frizzled4; Frizzled-4; Fz-4; FZD4; FZD4S; FzE4; GPCR; hFz4; MGC34390; WNT receptor frizzled-4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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