Human/Mouse Glypican 5 Fluorescein-conjugated Antibody

  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse Glypican 5 is observed and no cross‑reactivity with recombinant human Glypican 2, 3 or 6 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 297716
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Glypican 5
    Accession # P78333
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glypican 5 in H9 Human Cell Line by Flow Cytometry. H9 human cutaneous T lymphoma cell line was stained with Mouse Anti-Human/Mouse Glypican 5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2607F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glypican 5
Glypican 5 (GPC5) belongs to the Glypican family of heparan sulfate proteoglycans that are linked to the cell surface through a glycosyl-phosphatidylinositol anchor. It is synthesized as a glycosylated core protein of 63-65 kDa size that is further modified by both heparan and chondroitin sulfate adducts to create a 150-170 kDa glycosaminoglycan (GAG). Glypican 5 is principally expressed in neurons or neuronal precursors. As such, one role for Glypican 5 is to act as a co-receptor for SHH where it promotes SHH:Patched/PTC-1 interaction through its sulfated GAGs. Over amino acids 25-554, human and mouse Glypican 5 share 87% amino acid sequence identity.
  • Entrez Gene IDs:
    2262 (Human); 103978 (Mouse)
  • Alternate Names:
    bA93M14.1; glypican 5; glypican proteoglycan 5; glypican-5; GPC5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Fluorescein-conjugated Antibody

Ctrl IC003F 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse Glypican 5 Fluorescein-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information