Human/Mouse Glypican 5 Fluorescein-conjugated Antibody

FAB2607F has been discontinued. View all Glypican 5 products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse Glypican 5 is observed and no cross‑reactivity with recombinant human Glypican 2, 3 or 6 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 297716
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Glypican 5
    Glu25-Thr554
    Accession # P78333
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glypican 5 in H9 Human Cell Line by Flow Cytometry. H9 human cutaneous T lymphoma cell line was stained with Mouse Anti-Human/Mouse Glypican 5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2607F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glypican 5
Glypican 5 (GPC5) belongs to the Glypican family of heparan sulfate proteoglycans that are linked to the cell surface through a glycosyl-phosphatidylinositol anchor. It is synthesized as a glycosylated core protein of 63-65 kDa size that is further modified by both heparan and chondroitin sulfate adducts to create a 150-170 kDa glycosaminoglycan (GAG). Glypican 5 is principally expressed in neurons or neuronal precursors. As such, one role for Glypican 5 is to act as a co-receptor for SHH where it promotes SHH:Patched/PTC-1 interaction through its sulfated GAGs. Over amino acids 25-554, human and mouse Glypican 5 share 87% amino acid sequence identity.
  • Entrez Gene IDs:
    2262 (Human); 103978 (Mouse)
  • Alternate Names:
    bA93M14.1; Glypican 5; glypican proteoglycan 5; glypican-5; GPC5
Related Research Areas

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