Human/Mouse Glypican 5 PE‑conjugated Antibody

R&D Systems | Catalog # FAB2607P

R&D Systems
Discontinued Product
FAB2607P has been discontinued. View all Glypican 5 products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 297716
View all Glypican 5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glypican 5
Glu25-Thr554
Accession # P78333

Specificity

Detects human and mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse Glypican 5 is observed and no cross‑reactivity with recombinant human Glypican 2, 3 or 6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse Glypican 5 PE‑conjugated Antibody

Detection of Glypican 5 antibody in H9 Human Cell Line antibody by Flow Cytometry.

Detection of Glypican 5 in H9 Human Cell Line by Flow Cytometry.

H9 human cutaneous T lymphoma cell line was stained with Mouse Anti-Human/Mouse Glypican 5 PE-conjugated Monoclonal Antibody (Catalog # FAB2607P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse Glypican 5 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: H9 human cutaneous T lymphoma cell line

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Glypican 5

Glypican 5 (GPC5) belongs to the Glypican family of heparan sulfate proteoglycans that are linked to the cell surface through a glycosyl-phosphatidylinositol anchor. It is synthesized as a glycosylated core protein of 63-65 kDa size that is further modified by both heparan and chondroitin sulfate adducts to create a 150-170 kDa glycosaminoglycan (GAG). Glypican 5 is principally expressed in neurons or neuronal precursors. As such, one role for Glypican 5 is to act as a co-receptor for SHH where it promotes SHH:Patched/PTC-1 interaction through its sulfated GAGs. Over amino acids 25-554, human and mouse Glypican 5 share 87% amino acid sequence identity.

Alternate Names

GPC5

Entrez Gene IDs

2262 (Human); 103978 (Mouse)

Gene Symbol

GPC5

UniProt

P78333

Additional Glypican 5 Products

Product Documents for Human/Mouse Glypican 5 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Glypican 5 PE‑conjugated Antibody

For research use only

Related Research Areas

Proteoglycans
Wnt Signaling Modulators

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