Human/Mouse IGF-I R Antibody

(13 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human IGF-I R in sandwich ELISAs and Western blots. Detects mouse IGF-I R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL‑9 R, and rhTGF-beta  RII.
  • Source
    Monoclonal Mouse IgG1 Clone # 33255
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R
    Glu31-Asn932
    Accession # P08069
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
    • Human IGF-I R Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Mouse IGF‑I R Antibody (Catalog # MAB391)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IGF‑I R Biotinylated Antibody (Catalog # BAF391)
  • ELISA Standard
     
    Recombinant Human IGF-I R Protein, CF (Catalog # 391-GR)
  • Neutralization
    Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. At 11 µg/mL, this antibody will neutralize approximately 50-75% of the bioactivity due to 6 ng/mL Recombinant Human IGF‑I.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IGF‑I R by Western Blot.

Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, SK‑Mel‑28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IGF-I R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGF-I R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry
IGF‑I R in Mouse Heart. IGF‑I R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF‑I R Monoclonal Antibody (Catalog # MAB391) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF‑I R Antibody.

Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse IGF-I R Monoclonal Antibody (Catalog # MAB391). At 11 µg/mL, this antibody will neutralize 50-75% rhIGF-1 induced activity.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I R

Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

  • Long Name:
    Insulin-like Growth Factor I Receptor
  • Entrez Gene IDs:
    3480 (Human)
  • Alternate Names:
    CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes
    Authors: M Gschweitl, A Ulbricht, CA Barnes, RI Enchev, I Stoffel-St, N Meyer-Scha, J Huotari, Y Yamauchi, UF Greber, A Helenius, M Peter
    Elife, 2016;5(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.
    Authors: Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez-Villasenor I, Heath P, Romero I, Ince P, Wharton S
    Mol Brain, 2015;8(0):51.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators.
    Authors: Suh H, Zhao M, Derico L, Choi N, Lee S
    J Neuroinflammation, 2013;10(0):37.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Frozen
  4. Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro.
    Authors: Cardillo T, Trisal P, Arrojo R, Goldenberg D, Chang C
    BMC Cancer, 2013;13(0):170.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase (Nampt) involve regulation of insulin signaling pathway and Nampt enzymatic activity.
    Authors: Jacques C, Holzenberger M, Mladenovic Z
    J. Biol. Chem., 2012;287(18):15100-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Expression of the IGF axis is decreased in local prostate cancer but enhanced after benign prostate epithelial differentiation and TGF-beta treatment.
    Authors: Massoner P, Ladurner Rennau M, Heidegger I, Kloss-Brandstatter A, Summerer M, Reichhart E, Schafer G, Klocker H
    Am. J. Pathol., 2011;179(6):2905-19.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes.
    Authors: Back K, Arnqvist HJ
    Growth Horm. IGF Res., 2009;19(2):101-11.
    Species: Human
    Sample Type: Cell Lysates
    Application: ELISA Development
  8. Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases.
    Authors: Wang N, Fallavollita L, Nguyen L, Burnier J, Rafei M, Galipeau J, Yakar S, Brodt P
    Mol. Ther., 2009;17(7):1241-9.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  9. Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
    Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
    Leuk. Res., 2008;32(8):1244-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007;25(8):1862-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Mesenchymal stem cell abnormalities in patients with multiple myeloma.
    Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
    Leuk. Lymphoma, 2007;48(10):2032-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Insulin-like growth factor (IGF) 2 stimulates steroidogenesis and mitosis of bovine granulosa cells through the IGF1 receptor: role of follicle-stimulating hormone and IGF2 receptor.
    Authors: Spicer LJ, Aad PY
    Biol. Reprod., 2007;77(1):18-27.
    Species: Bovine
    Sample Type: Whole Cells
    Application: WB
  13. The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle.
    Authors: Quirk SM, Cowan RG, Harman RM
    J. Endocrinol., 2006;189(3):441-53.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 13 Citations
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