Human/Mouse LRRC4 Antibody

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Human/Mouse LRRC4 Antibody in Data
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Human/Mouse LRRC4 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse LRRC4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) LRRC3, rhLRRC4B, or rhLRRC32 is observed.
Monoclonal Mouse IgG2B Clone # 701424
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human LRRC4
Accession # Q9HBW1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse LRRC4 antibody by Western Blot. View Larger

Detection of Human and Mouse LRRC4 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LRRC4 Monoclonal Antibody (Catalog # MAB4995) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRRC4 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC4

LRRC4 (Leucine rich repeat/LRR-containing protein 4), also called NGL-2 (netrin-G ligand-2) or NAG14 (nasopharyngeal carcinoma-associated gene 14) is a 55 kDa (predicted, unglycosylated) type I transmembrane protein that is a member of the NGL family of synaptic LRR adhesion molecules (1, 2). Human LRRC4 cDNA encodes 653 amino acids (aa) that include a 38 aa signal sequence, a 489 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 105 aa cytoplasmic domain. The ECD contains nine LRRs (aa 74‑288), a C2 type Ig like domain (aa 354‑440), and a Thr-rich segment (aa 455‑526). Within the ECD, human LRRC4 shares 98% aa identity with mouse and rat, 99% aa identity with canine and bovine, and 99.6% aa identity with equine LRRC4. It also shares 54‑55% aa identity with family members LRRC4C/NGL-1 and LRRC4B/NGL-3, but each recognizes different ligands (1). LRRC4 is predominantly expressed in the brain on neurons and astrocytes as a ligand for netrin-G2 on the dendritic surface of synaptic neurons (2-4). It is proposed to regulate the formation of excitatory synapses via recruitment of PSD-95 to the cytoplasmic domain after aggregation of LRRC4 at the surface (3, 5). It suppresses proliferation by downregulating cell signaling pathways, resulting in altered expression of cell cycle regulating proteins and delay at the late G1 phase (1, 2, 6-8). It is thus considered a tumor suppressor protein and is often downregulated in brain tumors, particularly gliomas (1, 2, 6). Forced expression of LRRC4 in tumor cells slows proliferation and promotes differentiation (1, 4, 9). Addition of soluable LRRC4 to cultured neurons reduces excitatory synapse formation (3).

  1. Woo, J. et al. (2009) Mol. Cell. Neurosci. 42:1.
  2. Zhang, Q. et al. (2005) FEBS Lett. 579:3674.
  3. Kim, S. et al. (2006) Nat. Neurosci. 9:1294.
  4. Wu, M. et al. (2007) Acta Biochim Biophys Sin (Shanghai) 39:731.
  5. Nishimura-Akiyoshi, S. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14801.
  6. Wu, M. et al. (2006) Mol. Biol. Cell 17:3534.
  7. Wu, M. et al. (2008) J. Cell. Biochem. 103:245.
  8. Wu, M. et al. (2008) J. Cell. Physiol. 214:65.
  9. Zhang, W. et al. (2008) Genes Brain Behav. 7:385.
Long Name
Leucine-rich Repeat Containing 4
Entrez Gene IDs
64101 (Human); 192198 (Mouse); 641521 (Rat)
Alternate Names
BAG; Brain Tumor Associated Protein LRRC4; Brain Tumor-Associated Protein BAG; Leucine Rich Repeat Containing 4; Leucine-Rich Repeat-Containing 4; Leucine-Rich Repeat-Containing Protein 4; LRRC4; NAG14; Nasopharyngeal Carcinoma-Associated Gene 14 Protein; Netrin-G2 Ligand; NGL-2

Product Datasheets


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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