Human/Mouse Myeloperoxidase/MPO Antibody

Catalog # Availability Size / Price Qty
AF3667
AF3667-SP
Myeloperoxidase/MPO in Human Spleen.
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Product Details
Citations (22)
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Reviews (1)

Human/Mouse Myeloperoxidase/MPO Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MPO
Met16-Thr718
Accession # P11247
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Myeloperoxidase/MPO in Human Spleen. View Larger

Myeloperoxidase/MPO in Human Spleen. Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human/Mouse Myeloperoxidase/MPO by Western Blot. View Larger

Detection of Human/Mouse Myeloperoxidase/MPO by Western Blot. Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Myeloperoxidase/MPO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Mouse Myeloperoxidase/MPO by Simple WesternTM. View Larger

Detection of Mouse Myeloperoxidase/MPO by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Immunocytochemistry Myeloperoxidase/MPO in Mouse Splenocytes. View Larger

Myeloperoxidase/MPO in Mouse Splenocytes. Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

Entrez Gene IDs
4353 (Human); 17523 (Mouse); 303413 (Rat)
Alternate Names
EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase

Product Datasheets

Citations for Human/Mouse Myeloperoxidase/MPO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Qingnao dripping pills mediate immune-inflammatory response and MAPK signaling pathway after acute ischemic stroke in rats
    Authors: Z Zeng, Y Zhang, X Liang, F Wang, J Zhao, Z Xu, X Liu, X Liu
    J. Pharmacol. Sci., 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Tofacitinib enhances delivery of antibody-based therapeutics to tumor cells through modulation of inflammatory cells
    Authors: N Simon, A Antignani, SM Hewitt, M Gadina, C Alewine, D FitzGerald
    JCI Insight, 2019;0(0):.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Neutrophils escort circulating tumour cells to enable cell cycle progression
    Authors: BM Szczerba, F Castro-Gin, M Vetter, I Krol, S Gkountela, J Landin, MC Scheidmann, C Donato, R Scherrer, J Singer, C Beisel, C Kurzeder, V Heinzelman, C Rochlitz, WP Weber, N Beerenwink, N Aceto
    Nature, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in mice
    Authors: Z Cheng, L Wang, M Qu, H Liang, W Li, Y Li, L Deng, Z Zhang, GY Yang
    J Neuroinflammation, 2018;15(1):135.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Matrix metalloproteinase-9 deficiency protects mice from severe influenza A viral infection
    Authors: J Rojas-Quin, X Wang, J Tipper, PR Burkett, J Zuñiga, AR Ashtekar, F Polverino, A Rout, I Yambayev, C Hernández, L Jimenez, G Ramírez, KS Harrod, CA Owen
    JCI Insight, 2018;3(24):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation
    Authors: S Offenbache, Y Jiao, SJ Kim, J Marchesan, KL Moss, L Jing, K Divaris, S Bencharit, CS Agler, T Morelli, S Zhang, L Sun, WT Seaman, D Cowley, SP Barros, JD Beck, M Munz, AS Schaefer, KE North
    Nat Commun, 2018;9(1):3686.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis
    Authors: CWL Dougher, A Buffone, MJ Nemeth, M Nasirikena, EE Irons, PN Bogner, JTY Lau
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Cell-penetrating interactomic inhibition of nuclear factor-kappa B in a mouse model of postoperative cognitive dysfunction
    Authors: SY Cheon, JM Kim, EH Kam, CC Ho, EJ Kim, S Chung, JH Jeong, DD Lee, SW Lee, BN Koo
    Sci Rep, 2017;7(1):13482.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation
    Authors: M Toussaint, DJ Jackson, D Swieboda, A Guedán, TD Tsouroukts, YM Ching, C Radermecke, H Makrinioti, J Aniscenko, MR Edwards, R Solari, F Farnir, V Papayannop, F Bureau, T Marichal, SL Johnston
    Nat. Med., 2017;23(6):681-691.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Topical Fibronectin Improves Wound Healing of Irradiated Skin
    Authors: MB Johnson, B Pang, DJ Gardner, S Niknam-Ben, V Soundaraja, A Bramos, DP Perrault, K Banks, GK Lee, RY Baker, GH Kim, S Lee, Y Chai, M Chen, W Li, L Kwong, YK Hong, AK Wong
    Sci Rep, 2017;7(1):3876.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Obesity promotes prolonged ovalbumin-induced airway inflammation modulating Th1, Th2 and Th17 immune responses in BALB/c mice
    Authors: FM Silva, EE Oliveira, AC Gouveia, AS Brugiolo, CC Alves, JO Correa, J Gameiro, J Mattes, HC Teixeira, AP Ferreira
    Clin. Exp. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition
    PLoS Pathog, 2016;12(5):e1005644.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  13. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa
    PLoS Pathog., 2016;12(11):e1005987.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  15. PAD4 Deficiency Decreases Inflammation and Susceptibility to Pregnancy Loss in a Mouse Model
    Authors: Luise Erpenbeck
    Biol. Reprod, 2016;95(6):132.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
    PLoS ONE, 2015;10(3):e0119244.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Rhadinovirus host entry by co-operative infection.
    Authors: Lawler C, Milho R, May J, Stevenson P
    PLoS Pathog, 2015;11(3):e1004761.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
    Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
    Mucosal Immunol, 2014;7(5):1079-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Lactobacillus rhamnosus GG and Bifidobacterium longum attenuate lung injury and inflammatory response in experimental sepsis.
    Authors: Khailova L, Petrie B, Baird C, Dominguez Rieg J, Wischmeyer P
    PLoS ONE, 2014;9(5):e97861.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
    Authors: Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M
    Immunity, 2014;40(2):262-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Transcriptomic analysis of host immune and cell death responses associated with the influenza A virus PB1-F2 protein.
    Authors: Le Goffic R, Leymarie O, Chevalier C, Rebours E, Da Costa B, Vidic J, Descamps D, Sallenave JM, Rauch M, Samson M, Delmas B
    PLoS Pathog., 2011;7(8):e1002202.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Tissue inhibitor of metalloproteinases 3 regulates resolution of inflammation following acute lung injury.
    Authors: Gill SE, Huizar I, Bench EM
    Am. J. Pathol., 2010;176(1):64-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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Human/Mouse Myeloperoxidase/MPO Antibody
By Anonymous on 09/30/2018
Application: ICC/IF Sample Tested: Bone marrow cells Species: Mouse