Human/Mouse Myogenin Alexa Fluor® 488-conjugated Antibody Summary
Accession # P15173
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Myogenin in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse Myogenin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6686G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Myogenin, also known as MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the Myogenic Regulatory Factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa sequence identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.
Citations for Human/Mouse Myogenin Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Platelet-rich plasma, especially when combined with a TGF-beta inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro.
Authors: Kelc R, Trapecar M, Gradisnik L, Rupnik M, Vogrin M
PLoS ONE, 2015;10(2):e0117302.
Sample Types: Whole Cells
Applications: Flow Cytometry
Mesenchymal Stem Cells from Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
Authors: Boyle K, Patinkin Z, Shapiro A, Baker P, Dabelea D, Friedman J
Sample Types: Cell Culture Supernates
Applications: Flow Cytometry
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