Human/Mouse Myogenin Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6686G
Human/Mouse Myogenin Alexa Fluor® 488-conjugated Antibody in Data
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Citations (2)
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Human/Mouse Myogenin Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Myogenin in direct ELISAs and mouse Myogenin in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # 671038
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Myogenin antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Myogenin in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse Myogenin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6686G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Myogenin

Myogenin, also known as MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the Myogenic Regulatory Factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa sequence identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.

Entrez Gene IDs
4656 (Human); 17928 (Mouse); 29148 (Rat)
Alternate Names
BHLHC3; bHLHc3Myogenic factor-4; myogenin; Class C basic helix-loop-helix protein 3; MYF4; myf-4; MYF4myogenin; MYOG; Myogenic factor 4; myogenin (myogenic factor 4); Myogenin

Product Datasheets

Citations for Human/Mouse Myogenin Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Platelet-rich plasma, especially when combined with a TGF-beta inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro.
    Authors: Kelc R, Trapecar M, Gradisnik L, Rupnik M, Vogrin M
    PLoS ONE, 2015;10(2):e0117302.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mesenchymal Stem Cells from Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
    Authors: Boyle K, Patinkin Z, Shapiro A, Baker P, Dabelea D, Friedman J
    Diabetes, 0;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Flow Cytometry

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