Human/Mouse Oct-3/4 PerCP-conjugated Antibody

IC1759C has been discontinued. View all Oct-3/4 products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 240408
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Oct‑3/4
    Met1-Asn265 (Met262Leu)
    Accession # Q01860
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct‑3/4 PerCP‑conjugated Monoclonal Antibody (Catalog # IC1759C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of Oct‑3/4 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line treated with Retinoic Acid for 3 days was stained with Rat Anti-Human/Mouse Oct‑3/4 PerCP‑conjugated Monoclonal Antibody (Catalog # IC1759C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

  • References:
    1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
    2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
    3. Rosner, M.H. et al. (1990) Nature 345:686.
    4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
  • Long Name:
    Octamer-binding Protein 3/4
  • Entrez Gene IDs:
    5460 (Human); 18999 (Mouse)
  • Alternate Names:
    3-Oct; Oct-3/4; Oct4; Otf3g; Otf4; Pou5f1
Related Research Areas

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