Human/Mouse/Rat 14-3-3 beta Antibody

Catalog # Availability Size / Price Qty
AF4724
AF4724-SP

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Detection of Human/Mouse/ Rat 14‑3‑3 beta  by Western Blot.
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Human/Mouse/Rat 14-3-3 beta Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat 14-3-3 beta in Western blots. Does not cross-react with other recombinant human 14-3-3 isoforms.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human 14-3-3 beta
Met1-Asn246
Accession # P31946
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/ Rat 14-3-3 beta  antibody by Western Blot. View Larger

Detection of Human/Mouse/ Rat 14‑3‑3 beta by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line cells, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat 14-3-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recom-binant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (20 ng/lane) were included. A specific band for 14-3-3 beta was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 beta

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes - alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ.

Entrez Gene IDs
7529 (Human); 54401 (Mouse); 56011 (Rat)
Alternate Names
14-3-3 alpha; 1433 beta; 14-3-3 beta; 14-3-3 protein beta/alpha; GW128; HS1; YWHAB

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