Human/Mouse/Rat AIF Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    AIF antibodies are ideal for immunocytochemistry colocalization studies in mitochondria. The unconjugated antibody detects human, mouse, and rat AIF in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human AIF
    Glu121-Asp613
    Accession # O95831
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    1:10 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
AIF in HeLa Human Cell Line. AIF was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat AIF Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (green; Catalog # IC5824G) at 1:10 dilution overnight at 4 ° C and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: AIF
Apoptosis-inducing factor (AIF, also known as programmed cell death protein 8) is a 58 kDa member of the FAD-dependent oxidoreductase family of molecules. It is ubiquitously expressed and found in the mitochondrial intermembrane space. AIF likely acts as a mitochondrial antioxidant providing protection via NADH oxidase activity. Upon release from the mitochondria, AIF passes into the nucleus where it initiates apoptosis. Human AIF precursor is 67 kDa in size and 613 amino acids (aa) in length and contains a cleavable N-terminal 102 aa mitochondrial localization sequence, followed by a spacer region (aa 103‑129) and an oxidoreductase domain (aa 130‑613) that possesses an NLS (aa 446‑451). Over aa 121‑613, human AIF shares 95% aa identity with mouse AIF.
  • Long Name:
    Apoptosis Inducing Factor
  • Entrez Gene IDs:
    9131 (Human); 26926 (Mouse); 83833 (Rat)
  • Alternate Names:
    AIF; AIFapoptosis-inducing factor 1, mitochondrial; AIFM1; apoptosis-inducing factor, mitochondrion-associated, 1; COXPD6; MGC111425; PDCD8; PDCD8striatal apoptosis-inducing factor; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC016G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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