Human/Mouse/Rat Casein Kinase 1δ Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat Casein Kinase 1δ by Western Blot.
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Product Details
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Human/Mouse/Rat Casein Kinase 1δ Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse and rat CK1δ in Western blots. In Western blots, this antibody does not cross‑react with recombinant human CK1 alpha or rhCK1 gamma ; reactivity with CK1 epsilon is unknown.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human CK1δ
Accession # P48730
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat Casein Kinase 1δ by Western Blot. View Larger

Detection of Human/Mouse/Rat Casein Kinase 1δ by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1δ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1δ at approximately 49 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Immunocytochemistry Casein  Kinase 1δ in C2C12 Mouse Cell Line. View Larger

Casein Kinase 1δ in C2C12 Mouse Cell Line. Casein Kinase 1δ was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse/Rat Casein Kinase 1δ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Casein Kinase 1 delta

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3  and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1δ phosphorylates p53 as well as MDM2 and seems to play a principal role in coordinating the effects of these two key regulator proteins on cell growth and genome integrity.

Entrez Gene IDs
1453 (Human); 104318 (Mouse); 64462 (Rat)
Alternate Names
Casein Kinase 1 delta; casein kinase 1, delta; casein kinase I isoform delta; CKId; CKI-delta; CSNK1D; EC 2.7.11; EC; HCKID

Product Datasheets


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