|Detection of Human ILKAP/PP2Cδ by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cδ Monoclonal Antibody (Catalog # MAB4554) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILKAP/PP2Cδ at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cδ, is a serine/threonine phosphatase that is stimulated by manganese but inhibited by magnesium ions and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in liver, and skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.
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