Human/Mouse/Rat ILKAP/PP2Cδ Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human ILKAP in Western blots. In direct ELISAs, this antibody shows weak cross-reactivity with PP2C alpha (4%), PP2C beta (6%), and PP2C gamma (1%).
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ILKAP
    Met1-His392
    Accession # Q9H0C8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat ILKAP/PP2Cδ by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4554) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ILKAP/PP2C delta at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ILKAP/PP2C delta

Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cdelta, is a serine/threonine phosphatase that is stimulated by Mn2+ ions but inhibited by Mg2+ and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.

  • Long Name:
    Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C
  • Entrez Gene IDs:
    80895 (Human); 67444 (Mouse); 64538 (Rat)
  • Alternate Names:
    DKFZp434J2031; EC 3.1.3.16; FLJ10181; ILKAP; integrin-linked kinase-associated serine/threonine phosphatase 2C; integrin-linked kinase-associated serine/threonine phosphatase; MGC4846; PP2C delta; PP2C-DELTA; protein phosphatase 2c, delta isozyme
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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