Detects human, mouse, and rat Neurexophilin-3/NXPH-3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neurexophilin-1/NXPH1 and recombinant rat Neurexophilin-1/NXPH1 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant rat Neurexophilin-3/NXPH-3 Gln23-Gly252 Accession # AAI66603
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Neurexophilin‑3/NXPH‑3 by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Neurexophilin‑3/NXPH‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5098) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neurexophilin‑3/NXPH‑3 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Neurexophilins (NXPH) are a family of neuropeptide-like secreted glycoproteins expressed in specific regions of the brain. NXPH1 is expressed mainly by cells that resemble inhibitory interneurons, while NXPH3 is found selectively in subplate-derived neurons in cortical layer 6b, granule cells in the vestibulocerebellum and Cajal-Retzius cells during development. NXPH1, 2 and 3 are tightly bound extracellular ligands of alpha-neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release. NXPH2 is not expressed in rodents, and NXPH4 does not bind alpha-neurexins.
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