Detects rat Neuropilin-2 in direct ELISAs and detects human, mouse, and rat Neuropilin-2 in Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human Neuropilin‑1.
Monoclonal Mouse IgG2B Clone # 96009
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2 Gln23-Asp857 (Val809-Asp825 del) Accession # O35276
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human, Mouse, and Rat Neuropilin‑2 by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Neuropilin‑2 Monoclonal Antibody (Catalog # MAB567) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Neuropilin‑2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Neuropilin-2 in Rat Cortical Stem Cells by Flow Cytometry.
Rat cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat Neuropilin‑2 Monoclonal Antibody (Catalog # MAB567, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of Rat Neuropilin‑2 by Simple WesternTM.
Simple Western lane view shows lysates of C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin‑2 at approximately 139 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Neuropilin‑2 Monoclonal Antibody (Catalog # MAB567) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Neuropilin-1 (Npn-1, previously known as neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely non-overlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF165. Npn-1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.
Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
Neufeld, G. et al. (1999) FASEB J. 13:9.
Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.
Have you used Human/Mouse/Rat Neuropilin-2 Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.