Human/Mouse/Rat Sortilin Antibody

Catalog # Availability Size / Price Qty
MAB3154
MAB3154-SP
Detection of Human Sortilin by Western Blot.
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Product Details
Citations (1)
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Human/Mouse/Rat Sortilin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Sortilin in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 334708
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Sortilin
Ser78-Asn755
Accession # Q99523
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25-1 µg/mL
See below
Simple Western
10 µg/mL
See below
Knockout Validated
Sortilin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Sortilin knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Sortilin by Western Blot. View Larger

Detection of Human Sortilin by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human Sortilin by Simple WesternTM. View Larger

Detection of Human Sortilin by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 87 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        

Western Blot Detection  of Mouse and Rat Sortilin by Western Blot. View Larger

Detection of Mouse and Rat Sortilin by Western Blot. Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated Western  Blot Shows Human Sortilin Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Sortilin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Sortilin knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sortilin

Sortilin, also known as neurotensin receptor 3, is a 100 kDa transmembrane receptor that is localized both in the endosomal compartment and on plasma membranes. It contains an extracellular/lumenal Vps10p (vacuolar protein-sorting 10 protein) domain and is one of five known members of the mammalian Vps10p-D receptor family. A Sortilin propeptide, cleaved by a furin, blocks premature ligand binding to uncleaved Sortilin and functions as a chaperone to facilitate trafficking in the Golgi compartments. Sortilin binds multiple ligands and has diverse functions. Mature human Sortilin extracellular domain shares 92% and 91% amino acid sequence identity with that of mouse and rat Sortilin, respectively.

Entrez Gene IDs
6272 (Human); 20661 (Mouse)
Alternate Names
100 kDa NT receptor; Glycoprotein 95; Gp95; Gp95LDLCQ6; Neurotensin receptor 3; NT3NTR3; Ntr3; SORT1; sortilin 1; Sortilin

Product Datasheets

Citation for Human/Mouse/Rat Sortilin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A proteomic survey indicates sortilin as a secondary substrate of the ER translocation inhibitor cyclotriazadisulfonamide (CADA)
    Authors: Victor Van Puyenb
    Mol. Cell Proteomics, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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