Human/Mouse/Rat Substance P Antibody

Catalog # Availability Size / Price Qty
MAB4375
MAB4375-SP
Substance P in Human Spinal Cord.
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Product Details
Citations (5)
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Human/Mouse/Rat Substance P Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Substance P.
Source
Monoclonal Mouse IgG1 Clone # 266815
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH-coupled mouse Substance P synthetic peptide
RPKPQQFFGLM
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Substance P in Human Spinal Cord. View Larger

Substance P in Human Spinal Cord. Substance P was detected in immersion fixed paraffin-embedded sections of human spinal cord using 8 µg/mL Human/Mouse/Rat Substance P Monoclonal Antibody (Catalog # MAB4375) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Substance P

Substance P (Neurokinin-1), is member of the tachykinin peptide family. Processing of preprotachykinin isoforms generates Substance P and other tachykinins. Active Substance P is an 11 amino acid peptide, RPKPQQFFGLM, that is identical across all mammalian species. Substance P is mainly secreted from C-type fibers of primary sensory afferent neurons after stimulation through protease-activated receptor 2 (PAR2). Substance P binds primarily to the neurokinin 1 receptor on neurons in the dorsal horn, transmitting pain signals through the central nervous system.

Entrez Gene IDs
6863 (Human)
Alternate Names
neurokinin 1; neurokinin 2; neurokinin A; Neurokinin-1; neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma); neuropeptide gamma; neuropeptide K; NK2; NKA; NKNAneurokinin alpha; NPK; PPT; protachykinin-1; substance K; Substance P; TAC1; TAC2neuromedin L; tachykinin 2; tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2; tachykinin, precursor 1

Product Datasheets

Citations for Human/Mouse/Rat Substance P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model
    Authors: Y Xu, C Huang, H Deng, J Jia, Y Wu, J Yang, W Tu
    Turk J Gastroenterol, 2018;29(6):692-700.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. The dual regulation of substance P-mediated inflammation via human synovial mast cells in rheumatoid arthritis
    Authors: Y Okamura, S Mishima, JI Kashiwakur, T Sasaki-Sak, S Toyoshima, K Kuroda, S Saito, Y Tokuhashi, Y Okayama
    Allergol Int, 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Curcumin alleviates lumbar radiculopathy by reducing neuroinflammation, oxidative stress and nociceptive factors
    Authors: L Xiao, M Ding, A Fernandez, P Zhao, L Jin, X Li
    Eur Cell Mater, 2017;33(0):279-293.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Target-derived neurotrophins coordinate transcription and transport of bclw to prevent axonal degeneration.
    Authors: Cosker, Katharin, Pazyra-Murphy, Maria F, Fenstermacher, Sara J, Segal, Rosalind
    J Neurosci, 2013;33(12):5195-207.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Expression and distribution of mTOR, p70S6K, 4E-BP1, and their phosphorylated counterparts in rat dorsal root ganglion and spinal cord dorsal horn.
    Authors: Xu JT, Zhao X, Yaster M, Tao YX
    Brain Res., 2010;1336(0):46-57.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Secondary Antibodies

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