Human/Mouse Slit2 APC-conjugated Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Stains human and mouse Slit2 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Recombinant Monoclonal Rat IgG2B Clone # 710305
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Slit2
    Gln26-Gln900
    Accession # Q9R1B9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Slit2 in CHO Chinese Hamster Cell Line Transfected with human Slit2 by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with human Slit2 was stained with Rat Anti-Human/Mouse Slit2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5444A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Slit2 in CHO Chinese Hamster Cell Line Transfected with mouse Slit2 by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with mouse Slit2 was stained with Rat Anti-Human/Mouse Slit2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5444A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Slit2
Slit2 is a 180-210 kDa secreted member of the SLIT family of proteins. It is expressed in select sites in the embryo (via glia, motoneurons and posterior sclerotome mesoderm), and found in discrete adult cell types such as preosteoblasts, monocytes, granulosa lutein cells, and likely keratinocytes. Slit2 binds to multiple receptors, including ROBO-1 thru -4, laminin-1, Dan, Gremlin and netrin-1. Depending upon the target, Slit2 can promote a number of diverse effects, including both growth cone collapse and outgrowth, inhibition of dendritic cell migration, and axon repulsion. Mature mouse Slit2 is 1496 amino acids (aa) in length (aa 26-1521). It contains multiple intermingled domains, including nine EGF-like domains, 20 Leu-rich repeats (LRRs), one laminin G-like and CTCK (C-terminal Cys knot-like) domain, and eight total C-terminal plus N-terminal LRRs. There are two potential isoform splice variants. One contains a four aa insertion after Ser258, while another possesses the same insertion after Ser258 coupled to both an eight aa insertion after Ser479 and a nine aa insertion after Thr1021. Slit2 apparently undergoes proteolytic cleavage after Arg1113. This generates a 140-150 kDa N-terminal protein, and a 55-60 kDa C-terminal fragment. This processing does not inactivate Slit2. Rather, it creates molecules with distinct activities. For example, the N-terminal fragment will bind ROBO-1 and repel motor axon migration, while the C-terminal fragment won't bind ROBO-1, but will bind glypican-1 and promote motor axon migration. Over aa 26-900, mouse SLIT2 shares 99% and 97%aa sequence identity with rat and human Slit2, respectively.
  • Long Name:
    SLIT Homolog 2
  • Entrez Gene IDs:
    9353 (Human); 20563 (Mouse); 360272 (Rat)
  • Alternate Names:
    FLJ14420; SLIL3; slit (Drosophila) homolog 2; slit homolog 2 (Drosophila); slit homolog 2 protein; Slit2; Slit-2SLIL3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse Slit2 APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Customers Who Viewed
This Item Also Viewed

Feedback

Customer Information