Human/Mouse SSEA-4 PerCP-conjugated Antibody

FAB1435C has been discontinued. View all SSEA-4 products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
  • Source
    Monoclonal Mouse IgG3 Clone # MC-813-70
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    2120Ep human embryonal carcinoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SSEA‑4 in NTera‑2 human testicular embryonic carcinoma cell line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA‑4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB1435C, filled histogram) or isotype control antibody (Catalog # IC007C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

  • References:
    1. Shevinsky, L.H. et al. (1982) Cell 30:697.
    2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
    3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
    4. Draper, J.S. et al. (2002) J. Anat. 200:249.
  • Long Name:
    Stage-specific Embryonic Antigen-4
  • Alternate Names:
    SSEA4; SSEA-4
Related Research Areas

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