Human/Mouse Wnt-3a PE-conjugated Antibody

IC1324P has been discontinued. View all Wnt-3a products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse Wnt-3a in direct ELISAs and human and mouse Wnt-3a in Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse Wnt-1, -4, or 5a.
  • Source
    Monoclonal Rat IgG2A Clone # 217804
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Wnt‑3a
    Ser36-Gln75 and Trp219-Arg269
    Accession # P27467.1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Wnt‑3a in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Human/Mouse Wnt‑3a PE‑conjugated Monoclonal Antibody (Catalog # IC1324P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of Wnt‑3a in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Wnt‑3a PE‑conjugated Monoclonal Antibody (Catalog # IC1324P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Wnt-3a

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1-3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density Lipoprotein Receptor-related Protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).

  • References:
    1. Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.
    2. Miller, J.R. (2001) Genome Biol. 3:3001.
    3. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
    4. Lu, W et al. (2004) Cell 119:97.
    5. Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.
    6. Willert, K. et al. (2003) Nature 423:448.
    7. Komekado, H. et al. (2007) Genes Cells 12:521.
    8. Takada, R. et al. (2006) Dev. Cell 11:791.
    9. Entrez Accession # NP_001100475.
    10. Dunty, W.C. et al. (2008) Development 135:85.
    11. Lee, S.M.K. et al. (2000) Development 127:457.
    12. Takada, S. et al. (1994) Genes Dev. 8:174.
    13. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
    14. Nusse, R. (2008) Cell Res. 18:523.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 3a
  • Entrez Gene IDs:
    89780 (Human); 22416 (Mouse)
  • Alternate Names:
    MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a
Related Research Areas

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