Detects HEK293 human embryonic kidney cell line transfected with human MRGPRF by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Monoclonal Mouse IgG1 Clone # 916219
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human MRGPRF
Accession # NP_659452
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols
are available in the Technical Information section on our website.
Detection of MRGPRF in HEK293 Human Cell Line Transfected with Human MRGPRF and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human MRGPRF or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MRGPRF APC‑conjugated Monoclonal Antibody (Catalog # FAB8396A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
MRGPRF (MAS-related GPR, Member F), also known as RTA, MRGF, GPR140 or GPR168, belongs to the G protein coupled receptor 1 family. MRGPRF may be upregulated during the process of CSF-1 or GM-CSF-induced human monocyte to macrophage differentiation (1).
- Hohenhaus D.M., et al. (2013) Immunobiology 218:1345.
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