Human MRGPRF APC-conjugated Antibody

FAB8396A has been discontinued. View all MRGPRF products.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects HEK293 human embryonic kidney cell line transfected with human MRGPRF by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
  • Source
    Monoclonal Mouse IgG1 Clone # 916219
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human MRGPRF
    Met1-Ser343
    Accession # NP_659452
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of MRGPRF in HEK293 Human Cell Line Transfected with Human MRGPRF and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human MRGPRF or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MRGPRF APC‑conjugated Monoclonal Antibody (Catalog # FAB8396A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MRGPRF
MRGPRF (MAS-related GPR, Member F), also known as RTA, MRGF, GPR140 or GPR168, belongs to the G protein coupled receptor 1 family. MRGPRF may be upregulated during the process of CSF-1 or GM-CSF-induced human monocyte to macrophage differentiation (1).
  • References:
    1. Hohenhaus D.M., et al. (2013) Immunobiology 218:1345.

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