Human Myocilin Antibody

Catalog # Availability Size / Price Qty
MAB3446
MAB3446-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human Myocilin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Myocilin in direct ELISAs and Western blots. In direct ELISAs, 75% cross-reactivity with recombinant mouse Myocilin is observed.
Source
Monoclonal Mouse IgG2B Clone # 297817
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Myocilin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myocilin

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.

Entrez Gene IDs
4653 (Human)
Alternate Names
GLC1A; GLC1Amyocilin; GPOA; JOAG; JOAG1; MYOC; Myocilin; myocilin, trabecular meshwork inducible glucocorticoid response; TIGR; TIGRmutated trabecular meshwork-induced glucocorticoid response protein; Trabecular meshwork-induced glucocorticoid response protein

Product Datasheets

Citations for Human Myocilin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between�benign and glaucoma-causing mutations
    Authors: SE Hill, MS Kwon, MD Martin, A Suntharali, A Hazel, CA Dickey, JC Gumbart, RL Lieberman
    J. Biol. Chem., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Glucose-regulated protein 94 triage of mutant myocilin through endoplasmic reticulum-associated degradation subverts a more efficient autophagic clearance mechanism.
    Authors: Suntharalingam A, Abisambra J, O'Leary J, Koren J, Zhang B, Joe M, Blair L, Hill S, Jinwal U, Cockman M, Duerfeldt A, Tomarev S, Blagg B, Lieberman R, Dickey C
    J Biol Chem, 2012;287(48):40661-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  3. Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis.
    Authors: Joe MK, Tomarev SI,
    Am. J. Pathol., 2010;176(6):2880-90.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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