Human Myocilin Antibody Summary
Accession # Q99972
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Myocilin by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Myocilin at approximately 55 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Myocilin by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Myocilin at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 amino acid (aa) precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha -helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat and mouse.
Citations for Human Myocilin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle
Authors: JM Lynch, AJ Dolman, C Guo, K Dolan, C Xiang, S Reda, B Li, G Prasanna
PLoS ONE, 2018;13(11):e0206801.
Sample Types: Cell Culture Supernate
Binding of a glaucoma-associated myocilin variant to the ?B-crystallin chaperone impedes protein clearance in trabecular meshwork cells
Authors: JM Lynch, B Li, P Katoli, C Xiang, B Leehy, N Rangaswamy, V Saenz-Vash, YK Wang, H Lei, TB Nicholson, E Meredith, D Rice, G Prasanna, A Chen
J. Biol. Chem., 2018;0(0):.
Sample Types: Aqueous Humor
Reduced myocilin expression in cultured monkey trabecular meshwork cells induced by a selective glucocorticoid receptor agonist: comparison with steroids.
Authors: Pfeffer BA, DeWitt CA, Salvador-Silva M, Cavet ME, Lopez FJ, Ward KW
Invest. Ophthalmol. Vis. Sci., 2010;51(1):437-46.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Culture Supernates
Substrate rigidity modulates cell matrix interactions and protein expression in human trabecular meshwork cells.
Authors: Schlunck G, Han H, Wecker T, Kampik D, Meyer-ter-Vehn T, Grehn F
Invest. Ophthalmol. Vis. Sci., 2008;49(1):262-9.
Sample Types: Whole Cells
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