Human Myocilin Antibody

Catalog # Availability Size / Price Qty
AF2537
AF2537-SP
Detection of Human Myocilin by Western Blot.
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Product Details
Citations (4)
FAQs
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Human Myocilin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Myocilin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Myocilin by Western Blot. View Larger

Detection of Human Myocilin by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Myocilin at approximately 55 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Myocilin by Simple WesternTM. View Larger

Detection of Human Myocilin by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Myocilin at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myocilin

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 amino acid (aa) precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha -helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat and mouse.

Entrez Gene IDs
4653 (Human)
Alternate Names
GLC1A; GLC1Amyocilin; GPOA; JOAG; JOAG1; MYOC; Myocilin; myocilin, trabecular meshwork inducible glucocorticoid response; TIGR; TIGRmutated trabecular meshwork-induced glucocorticoid response protein; Trabecular meshwork-induced glucocorticoid response protein

Product Datasheets

Citations for Human Myocilin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle
    Authors: JM Lynch, AJ Dolman, C Guo, K Dolan, C Xiang, S Reda, B Li, G Prasanna
    PLoS ONE, 2018;13(11):e0206801.
    Species: Human
    Sample Types: Cell Culture Supernate
    Applications: WB
  2. Binding of a glaucoma-associated myocilin variant to the ?B-crystallin chaperone impedes protein clearance in trabecular meshwork cells
    Authors: JM Lynch, B Li, P Katoli, C Xiang, B Leehy, N Rangaswamy, V Saenz-Vash, YK Wang, H Lei, TB Nicholson, E Meredith, D Rice, G Prasanna, A Chen
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Aqueous Humor
    Applications: WB
  3. Reduced myocilin expression in cultured monkey trabecular meshwork cells induced by a selective glucocorticoid receptor agonist: comparison with steroids.
    Authors: Pfeffer BA, DeWitt CA, Salvador-Silva M, Cavet ME, Lopez FJ, Ward KW
    Invest. Ophthalmol. Vis. Sci., 2010;51(1):437-46.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates
    Applications: WB
  4. Substrate rigidity modulates cell matrix interactions and protein expression in human trabecular meshwork cells.
    Authors: Schlunck G, Han H, Wecker T, Kampik D, Meyer-ter-Vehn T, Grehn F
    Invest. Ophthalmol. Vis. Sci., 2008;49(1):262-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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