Human NAALADase-like 2/NAALADL2 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4665A
Detection of NAALADase‑like 2/NAALADL2 in LNCaP Human Cell Line by Flow Cytometry.
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Human NAALADase-like 2/NAALADL2 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NAALADase‑like 2/NAALADL2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 817225
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NAALADase‑like 2/NAALADL2
Ser152-Asn795
Accession # Q58DX5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of NAALADase‑like 2/NAALADL2 in LNCaP Human Cell Line by Flow Cytometry. View Larger

Detection of NAALADase‑like 2/NAALADL2 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human NAALADase‑like 2/NAALADL2 APC‑conjugated Monoclonal Antibody (Catalog # FAB4665A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NAALADase-like 2/NAALADL2

NAALADL2 (N-acetylated alpha -linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa 688‑777). There are multiple splice forms. One shows a 4 aa substitution for aa 292‑795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314‑795, and a third contains an alternate start site at Met283, accompanied by a deletion of aa 363‑411 and 633‑795. Over aa 152‑795, human NAADADL2 shares 87% and 82% aa sequence identity with mouse and canine NAADADL2, respectively.

Long Name
N-Acetylated Alpha-Linked Acidic Dipeptidase-like 2
Entrez Gene IDs
254827 (Human); 635702 (Mouse); 499583 (Rat)
Alternate Names
inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2; NAALADase L2; NAALADase like 2; NAALADase-like 2; NAALADL2; N-acetylated alpha-linked acidic dipeptidase 2; N-acetylated alpha-linked acidic dipeptidase-like 2

Product Datasheets

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