Human Nectin-3 Antibody

Catalog # Availability Size / Price Qty
AF3064
AF3064-SP
Human Nectin-3 Antibody in Immunohistochemistry
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human Nectin-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nectin-3 in direct ELISAs and Western blots. In direct ELISAs, less than 65% cross-reactivity with recombinant mouse
Nectin-3 is observed and less than 5% cross-reactivity with recombinant human (rh) Nectin-1, rhNectin-2, and rhNectin-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin-3
Leu56-Asp400
Accession # Q9NQS3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Nectin‑3 (Catalog # 3064-N3)
Flow Cytometry
2.5 µg/106 cells
HepG2 human hepatocellular carcinoma cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Nectin‑3 in Human Kidney Array. View Larger

Nectin‑3 in Human Kidney Array. Nectin‑3 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human Nectin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3064) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-3

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1-4). The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids. The ECD shows three Ig-like domains; one N-terminal V-type and two membrane-proximal C2-type. The cytoplasmic region shows a Glu-Trp-Tyr-Val motif that binds afadin (3, 5, 6). The ECD of human Nectin-3 is 94% aa identical to mouse Nectin-3 ECD (5, 6). Nectin-3 has a diverse expression pattern. It has been found in junctions between small intestinal columnar epithelial cells (6), pigmented and nonpigmented epithelium in the ciliary body (7), spermatids and Sertoli cells in the seminiferous tubules (8), and on spinal cord motor neurons and axons, plus Schwann cells of the peripheral nervous system (9). As with mouse, human Nectin-3 has three potential isoforms. It does not appear that they are strict orthologs. In addition to the full length isoform, a second human isoform has a 31 aa substitution for the first 54 aa of the signal sequence, followed by a deletion of aa 291-549 (10). The third human isoform shows a 10 aa substitution for aa 357-549 (11). Nectin-3 is proposed to promote cell adhesion by initiating cell-to-cell adhesion, allowing for subsequent JAM and cadherin-based intercellular junction formation. It does so by first forming same cell (cis-) heterodimers with alpha v beta 3 and PDGF R (an anti-apoptotic interaction) (2, 4). This results in actin reorganization and recruitment of adherens and occludins adhesion molecules. Subsequent Nectin-3 activity adds strength to the junction through trans-interactions with a variety of molecules, including Nectin-3 itself, as well as Nectins-1 and 2 plus Necl-1, 2 and 5 (3, 4).

References
  1. Takai, Y. and H. Nakanishi (2003) J. Cell Sci. 116:17.
  2. Miyoshi, J. and Y. Takai (2007) Am. J. Nephrol. 27:590.
  3. Sakisaka, T. et al. (2007) Curr. Opin. Cell Biol. 19:593.
  4. Rikitake, Y. and Y. Takai (2008) Cell. Mol. Life Sci. 65:253.
  5. Reymand, N. et al. (2000) Gene 255:347.
  6. Satoh-Horikawa, K. et al. (2000) J. Biol. Chem. 275:10291.
  7. Inagaki, M. et al. (2005) Development 132:1525.
  8. Inagaki, M. et al. (2006) Genes Cells 11:1125.
  9. Zelano, J. et al. (2006) Exp. Neurol. 201:461.
  10. GenBank Accession # BAC11414.
  11. SwissProt # Q9NQS3.
Long Name
Poliovirus Receptor Related 3
Entrez Gene IDs
25945 (Human); 58998 (Mouse); 288124 (Rat)
Alternate Names
CD113 antigen; CD113; CDw113FLJ90624; DKFZp566B0846; nectin 3; Nectin3; Nectin-3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; PPR3; PRR3CD113; PVRL3; PVRR3

Product Datasheets

Citations for Human Nectin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027
    Authors: Z Peng, R Simeon, SB Mitchell, J Zhang, H Feng, Z Chen
    mSphere, 2019;4(5):.
    Species: Human
    Sample Types: Protein
    Applications: ELISA (Capture)
  2. CADM1 is a key receptor mediating human mast cell adhesion to human lung fibroblasts and airway smooth muscle cells.
    Authors: Moiseeva, Elena P, Roach, Katy M, Leyland, Mark L, Bradding, Peter
    PLoS ONE, 2013;8(4):e61579.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Nectin-3 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Nectin-3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Nectin-3 Antibody
By Anonymous on 11/30/2018
Application: ICC/IF Sample Tested: c2bbe Species: Human