Detection of Nectin‑4 in MCF‑7 Human Cell Line by Flow Cytometry.
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Nectin‑4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2659C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Nectin-4 is a 66-70 kDa member of the nectin family, Ig superfamily of molecules. Mature Nectin-4 is a 479 amino acid (aa) Type I transmembrane glycoprotein that contains one V-type and two C2-type Ig-like domains. Nectins mediate the Ca++-independent cell adhesion in junctional complexes and connect to the Actin cytoskeleton via Afadin. Nectins are considered to first form loose cis-homodimers which aggregate and generate homooligomer in-trans, but preferentially generates Nectin-4:Nectin-1 heterooligomers. Cells known to express Nectin-4 include keratinocytes, transitional epithelium, CD49f+ CD24+ luminal mammary epithelium, tooth stratum intermedium cells, and respiratory pseudostratified ciliated columnar epithelium. It is also found on multiple carcinoma cell types. Nectin-4 can be cleaved to generate a 43-44 kDa soluble form that apparently appears only during tumorigenesis. Nectin-4 is also known to bind the measles virus, and possibly interact, indirectly or as a heterodimer, with the prolactin receptor. Over aa 27-351, human and mouse Nectin-4 share 90% aa sequence identity.
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