Human Neuritin Alexa Fluor® 647-conjugated Antibody

FAB283R has been discontinued. View all Neuritin products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Neuritin in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse Neuritin.
  • Source
    Monoclonal Mouse IgG2B Clone # 79537
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neuritin
    Ala28-Gly114
    Accession # Q9NPD7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Neuritin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Neuritin Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB283R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Neuritin
Neuritin (NRN1) is a 14 kDa GPI-linked molecule that is expressed on developing and differentiating neurons, as well as hepatocytes and Schwann cells. Neuritin is upregulated in response to BDNF, NT-3, androgens, and hypoxia, and it promotes neuronal migration, neurite extension, and arborization. Neuritin does contribute to the formation of the ionotrophic glutamate receptor APMA. Here, it is a component of the outer core. Mature human Neuritin shares 99% and 100% amino acid sequence identity with mouse and rat Neuritin, respectively.
  • Entrez Gene IDs:
    51299 (Human)
  • Alternate Names:
    dJ380B8.2; neuritin 1; Neuritin; NRN1; NRNMGC44811
Related Research Areas

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