Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.
Monoclonal Mouse IgG1 Clone # 74902
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R Lys29-Asn250 Accession # P08138
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of NGF R/TNFRSF16 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 Biotinylated Monoclonal Antibody (Catalog # FAB367B, filled histogram) or isotype control antibody (Catalog # IC002B, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: NGF R/TNFRSF16
NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).
Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
Stemple, D.L. et al. (1992) Cell 71:973.
Morrison, S.J. et al. (1999) Cell 96:737.
Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.