Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.
Monoclonal Mouse IgG1 Clone # 74902
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R Lys29-Asn250 Accession # P08138
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of NGF R/TNFRSF16 in SH‑SY5Y Human Cell Line by Flow Cytometry.
SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 PE‑conjugated Monoclonal Antibody (Catalog # FAB367P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: NGF R/TNFRSF16
NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).
Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
Stemple, D.L. et al. (1992) Cell 71:973.
Morrison, S.J. et al. (1999) Cell 96:737.
Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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