Human NGF R/TNFRSF16 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 74902
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R
    Accession # P08138
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NGF R/TNFRSF16 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 PE‑conjugated Monoclonal Antibody (Catalog # FAB367P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NGF R/TNFRSF16
NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).
  • References:
    1. Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
    2. Stemple, D.L. et al. (1992) Cell 71:973.
    3. Morrison, S.J. et al. (1999) Cell 96:737.
    4. Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
    5. Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
    6. Cassiman, D. et al. (2001) Hepatology 33:148.
    7. Long Name:
      Nerve Growth Factor Receptor
    8. Entrez Gene IDs:
      4804 (Human); 18053 (Mouse); 117089 (Rat)
    9. Alternate Names:
      CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16
    Related Research Areas
    Isotype Controls
    Description Application Cat# Citations Images  

    Mouse IgG1 PE-conjugated Antibody

    Ctrl IC002P 28  
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    Staining Reagents
    Description Application Cat# Citations Images  

    Flow Cytometry Staining Buffer (1X)

    Flow FC001 3
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    Flow Cytometry Mouse Lyse Buffer (10X)

    Flow FC003 1
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    Flow Cytometry Human Lyse Buffer (10X)

    Flow FC002 1
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