Human NKp46/NCR1 Antibody

Clone 195314 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1850-SP
MAB1850-100
MAB1850-500
NKp46/NCR1  in NK-92 Human Cell Line and Human PBMCs.
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Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (5)

Human NKp46/NCR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKp46/NCR1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 195314
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse T cell hybridoma transfected with human NKp46/NCR1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human NKp46/NCR1 Fc Chimera (Catalog # 1850-NK)
Flow Cytometry
2.5 µg/106 cells
Human whole blood CD56+ natural killer cells
Immunohistochemistry
5-25 µg/mL
See below
Agonist Activity
Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells.
The ED50 for this effect is typically 0.1‑0.3 μg/mL.
 
CyTOF-reported
Horowitz, A. et al. Sci. Transl. Med. (2013) 208ra145. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry NKp46/NCR1  in NK-92 Human Cell Line and Human PBMCs. View Larger

NKp46/NCR1 in NK-92 Human Cell Line and Human PBMCs. NKp46/NCR1 was detected in immersion fixed NK‑92 human natural killer lymphoma cell line (left panel, positive stain) and human peripheral blood mononuclear cells (PBMCs; right panel, negative stain) using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry NKp46/NCR1  in Human Liver. View Larger

NKp46/NCR1 in Human Liver. NKp46/NCR1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to natural killer cells in trabecules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

References
  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255. 
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. 
  4. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  5. Arnon, T. et al. (2004) Blood 103:664.
  6. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  7. Mandelboim, O. et al. (2001) Nature 409:1055.
  8. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.
Entrez Gene IDs
9437 (Human); 17086 (Mouse)
Alternate Names
CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

Product Datasheets

Citations for Human NKp46/NCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Mutated Von Hippel-Lindau-renal cell carcinoma (RCC) promotes patients specific natural killer (NK) cytotoxicity
    Authors: AM Trotta, S Santagata, S Zanotta, C D'Alterio, M Napolitano, G Rea, R Camerlingo, F Esposito, E Lamantia, A Anniciello, G Botti, N Longo, G Botti, S Pignata, S Perdonà, S Scala
    J. Exp. Clin. Cancer Res., 2018;37(1):297.
    Species: Human
    Sample Types: Tissue Section
    Applications: IHC
  2. Targeted therapies for targeted populations: Anti-EGFR treatment for EGFR amplified gastroesophageal adenocarcinoma
    Authors: SB Maron, L Alpert, HA Kwak, S Lomnicki, L Chase, D Xu, E O'Day, RJ Nagy, RB Lanman, F Cecchi, T Hembrough, A Schrock, J Hart, SY Xiao, N Setia, DVT Catenacci
    Cancer Discov, 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
    Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
    Sci Rep, 2017;7(0):44544.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  4. Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models
    Authors: Y Feng, S Liao, C Wei, D Jia, K Wood, Q Liu, X Wang, FD Shi, WN Jin
    J Neuroinflammation, 2017;14(1):248.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. NKp30 isoforms and NKp46 transcripts in metastatic melanoma patients: Unique NKp30 pattern in rare melanoma patients with favorable evolution
    Authors: M Messaouden, G Fregni, D Enot, N Jacquelot, E Neves, N Germaud, HJ Garchon, W Boukouaci, R Tamouza, J Chanal, MF Avril, A Toubert, L Zitvogel, S Rusakiewic, A Caignard
    Oncoimmunology, 2016;5(12):e1154251.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  6. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016;197(5):1621-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  7. Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection.
    Authors: Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z
    Immunity, 2016;45(1):131-44.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients.
    Authors: Messaoudene M, Perier A, Fregni G, Neves E, Zitvogel L, Cremer I, Chanal J, Sastre-Garau X, Deschamps L, Marinho E, Larousserie F, Maubec E, Avril M, Caignard A
    PLoS ONE, 2015;10(7):e0133363.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  9. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
    Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
    J Immunol, 2014;192(8):3805-15.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.
    Authors: Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss S, Kiprijanov S, Little M, Zhukovsky E
    MAbs, 2014;6(3):728-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  11. Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients.
    Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F
    PLoS ONE, 2012;7(11):e49135.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  12. Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1).
    Authors: Durrenberger PF, Ettorre A, Kamel F
    J Neuroinflammation, 2012;9(0):1.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Sequential metastases of colorectal cancer: Immunophenotypes and spatial distributions of infiltrating immune cells in relation to time and treatments.
    Authors: Keim S, Zoernig I, Spille A, Lahrmann B, Brand K, Herpel E, Grabe N, Jager D, Halama N
    Oncoimmunology, 2012;1(5):593-599.
  14. NKp46 identifies an NKT cell subset susceptible to leukemic transformation in mouse and human.
    Authors: Yu J, Mitsui T, Wei M, Mao H, Butchar JP, Shah MV, Zhang J, Mishra A, Alvarez-Breckenridge C, Liu X, Liu S, Yokohama A, Trotta R, Marcucci G, Benson DM, Loughran TP, Tridandapani S, Caligiuri MA
    J. Clin. Invest., 2011;121(4):1456-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  15. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008;181(3):2227-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  16. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007;111(3):1309-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. A novel binding assay to assess specificity of monoclonal antibodies.
    Authors: Warren HS, Warren&lt;/LastName&gt;&lt;ForeNam HS&lt;/Initia, Bettadapura J
    J. Immunol. Methods, 2005;305(1):33-8.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Epitope Mapping
  18. Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways.
    Authors: Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, Hong M, Bertoletti A, Bicciato S, Invernizzi P, Lugli E, Torzilli G, Gershwin M, Mavilio D
    J Autoimmun, 0;66(0):40-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Mouse IgG2B Antibody

Reviews for Human NKp46/NCR1 Antibody

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Human NKp46/NCR1 Antibody
By Anonymous on 05/18/2018
Application: IHC Sample Tested: Lymph node tissue Species: Human

Paraffin embedded lymph node metastasis samples
Antigen retrieval: Citrat buffer
Antibody Dilution: 1:50
Buffer: TBS-T

At this concentration, the isotype showed no specific staining, whereas MAB1850 showed clear surface staining of cells.


Human NKp46/NCR1 Antibody
By Anonymous on 10/01/2017
Application: ICC/IF Sample Tested: Kidney (cortex) tissue Species: Human

Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

At this concentration there is little backgroud.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:500


Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

Seems like the immune cells show a little staining. At 1:250 all cells showed staining. Dilutions are important.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000


Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: Natural killer cells Species: Human

Human NKp46/NCR1 MAb (Clone 195314), works well for detection of NKp46 on surface of NK cells.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:500