Human Olig2 Antibody

(26 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Olig2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Olig3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Olig2
    Met1-Lys323
    Accession # Q13516
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Olig2
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Chromatin Immunoprecipitation (ChIP)
    5 µg/5 x 106 cells
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Chromatin Immunoprecipitation (ChIP)
Detection of Olig2-regulated Genes by Chromatin Immunoprecipitation. A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
Immunohistochemistry
Olig2 in Mouse Embryo. Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Olig2
Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.
  • Long Name:
    Oligodendrocyte Lineage Transcription Factor 2
  • Entrez Gene IDs:
    10215 (Human)
  • Alternate Names:
    BHLHB1; BHLHE19; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; Olig2; OLIGO2; oligodendrocyte lineage transcription factor 2; oligodendrocyte transcription factor 2; oligodendrocyte-specific bHLH transcription factor 2; PRKCBP2; protein kinase C binding protein 2; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17; RACK17helix-loop-helix protein, class B, 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1
    Authors: FI Young, M Keruzore, X Nan, N Gennet, EJ Bellefroid, M Li
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors
    Authors: NJ Yates, MK Giacci, RL O'Hare Doi, W Chiha, BE Ashworth, J Kenna, CA Bartlett, M Fitzgerald
    Neural Regen Res, 2017;12(2):307-316.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  3. Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors
    Authors: SH Choi, SH Kim, KW Shim, JW Han, J Choi, DS Kim, CJ Lyu, JW Kim, CO Suh, J Cho
    PLoS ONE, 2016;11(4):e0153443.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation
    Authors: Qi Xie
    J Clin Invest, 2016;126(7):2757-2772.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Patched1 and Patched2 inhibit Smoothened non-cell autonomously
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  6. A cord blood monocyte-derived cell therapy product accelerates brain remyelination
    JCI Insight, 2016;1(13):e86667.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  7. Brain tissue banking for stem cells for our future
    Sci Rep, 2016;6(0):39394.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: IHC Not Specified
  8. Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP Assay
  9. Notch signaling regulates motor neuron differentiation of human embryonic stem cells.
    Authors: Ben-Shushan E, Feldman E, Reubinoff B
    Stem Cells, 2015;33(2):403-15.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  10. Mitochondrial control by DRP1 in brain tumor initiating cells.
    Authors: Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J
    Nat Neurosci, 2015;18(4):501-10.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair.
    Authors: Rzem R, Achouri Y, Marbaix E, Schakman O, Wiame E, Marie S, Gailly P, Vincent M, Veiga-da-Cunha M, Van Schaftingen E
    PLoS ONE, 2015;10(3):e0119540.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT
  12. BMP signaling induces astrocytic differentiation of clinically derived oligodendroglioma propagating cells.
    Authors: Srikanth, Maya, Kim, Juno, Das, Sunit, Kessler, John A
    Mol Cancer Res, 2014;12(2):283-94.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
    Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J
    Genes Dev, 2014;28(10):1085-100.
    Species: Human
    Sample Type: Whole Tissue
    Application: IF
  14. Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum.
    Authors: Seto Y, Nakatani T, Masuyama N, Taya S, Kumai M, Minaki Y, Hamaguchi A, Inoue Y, Inoue T, Miyashita S, Fujiyama T, Yamada M, Chapman H, Campbell K, Magnuson M, Wright C, Kawaguchi Y, Ikenaka K, Takebayashi H, Ishiwata S, Ono Y, Hoshino M
    Nat Commun, 2014;5(0):3337.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  15. Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells.
    Authors: Bergsland M, Covacu R, Perez Estrada C, Svensson M, Brundin L
    Stem Cells, 2014;32(9):2539-49.
    Species: Rat
    Sample Type: Whole Cells
    Application: IHC
  16. Phage display discovery of novel molecular targets in glioblastoma-initiating cells.
    Authors: Liu, J K, Lubelski, D, Schonberg, D L, Wu, Q, Hale, J S, Flavahan, W A, Mulkearns-Hubert, E E, Man, J, Hjelmeland, A B, Yu, J, Lathia, J D, Rich, J N
    Cell Death Differ, 2014;21(8):1325-39.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. Safety of epicenter versus intact parenchyma as a transplantation site for human neural stem cells for spinal cord injury therapy.
    Authors: Piltti K, Salazar D, Uchida N, Cummings B, Anderson A
    Stem Cells Transl Med, 2013;2(3):204-16.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  18. Protein tyrosine phosphatase receptor type z negatively regulates oligodendrocyte differentiation and myelination.
    Authors: Kuboyama, Kazuya, Fujikawa, Akihiro, Masumura, Makoto, Suzuki, Ryoko, Matsumoto, Masahito, Noda, Masaharu
    PLoS ONE, 2012;7(11):e48797.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  19. N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells.
    Authors: Martinez-Cerdeno V, Lemen JM, Chan V, Wey A, Lin W, Dent SR, Knoepfler PS
    PLoS ONE, 2012;7(6):e39456.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  20. The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Authors: Zelinka C, Scott M, Volkov L, Fischer A
    PLoS ONE, 2012;7(9):e44477.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC
  21. Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model.
    Authors: Salazar DL, Uchida N, Hamers FP, Cummings BJ, Anderson AJ
    PLoS ONE, 2010;5(8):e12272.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  22. Characterization of progenitor domains in the developing mouse thalamus.
    Authors: Vue TY, Aaker J, Taniguchi A
    J. Comp. Neurol., 2007;505(1):73-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  23. Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice.
    Authors: Widestrand A, Faijerson J, Wilhelmsson U, Smith PL, Li L, Sihlbom C, Eriksson PS, Pekny M
    Stem Cells, 2007;25(10):2619-27.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  24. Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain.
    Authors: Bauer S, Patterson PH
    J. Neurosci., 2006;26(46):12089-99.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  25. Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro.
    Authors: Mendes F, Coelho Aguiar J, Kahn S, Reis A, Dubois L, Romao L, Ferreira L, Chneiweiss H, Moura Neto V, Abreu J
    PLoS ONE, 0;10(8):e0133689.
    Species: Human
    Sample Type: Cell Lysates
    Application: NotWB
Expand to show all 26 Citations
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Isotype Controls
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Normal Goat IgG Control

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Average Rating: 5 (Based on 1 review)

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Summary

ApplicationAffinity Purification
Sample TestedBrain sections

Other Experimental Details

Other Experimental DetailsDilution 1:500

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