Human P2Y1/P2RY1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB9290P-100
Detection  of P2Y1/P2RY1 in HEK293 Human Cell Line Transfected with Human P2Y1/P2RY1 and  eGFP by Flow Cytometry.
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Human P2Y1/P2RY1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human P2Y1/P2RY1 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 479509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human cell line transfected with human P2Y1/P2RY1
Accession # NP_002554
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of P2Y1/P2RY1 in HEK293 Human Cell Line Transfected with Human P2Y1/P2RY1 and  eGFP by Flow Cytometry. View Larger

Detection of P2Y1/P2RY1 in HEK293 Human Cell Line Transfected with Human P2Y1/P2RY1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human P2Y1/P2RY1 and eGFP were stained with (A) Mouse Anti-Human P2Y1/P2RY1 PE-conjugated Monoclonal Antibody (Catalog # FAB9290P) or (B) isotype control antibody (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: P2Y1/P2RY1

P2RY1 belongs to the family of G protein-coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. P2RY1 functions as a receptor for extracellular ATP and ADP. In platelets, binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C. ADP binding also may lead to a change in platelet shape and possible platelet aggregation.

Long Name
P2Y Purinergic Receptor 1
Entrez Gene IDs
5028 (Human); 18441 (Mouse); 25265 (Rat)
Alternate Names
ATP receptor; P2 purinoceptor subtype Y1; P2RY1; P2Y purinoceptor 1; P2Y1; P2Y1platelet ADP receptor; purinergic receptor P2Y, G-protein coupled, 1; Purinergic receptor

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