Human p38 beta Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human p38 beta in direct ELISAs. No cross-reactivity with recombinant human p38 alpha, gamma, or delta is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 280071
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human p38 beta synthetic peptide
    EPPKPPGSLEIEQ
    Accession # NP_002742
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
p38 beta in Jurkat Human Cell Line. p38 beta was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human p38 beta Monoclonal Antibody (Catalog # MAB3274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 beta
The p38 mitogen-activated protein kinases (p38 MAP kinases) are a family of four related Ser/Thr kinases responsive to pro-inflammatory cytokines and environmental stresses, including ionizing radiation, oxidative stress, and osmotic shock. Each family member, p38 alpha, p38 beta, p38δ, and p38 gamma, is activated by dual Thr and Tyr phosphorylation within a Thr-Gly-Tyr motif residing in the kinase activation loop. For p38 beta, also known as p38-2, stress-activated protein kinase 2B (SAPK2B), and MAPK11, this dual phosphorylation occurs at Thr180/Tyr182.
  • Entrez Gene IDs:
    5600 (Human); 19094 (Mouse)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.24; MAP kinase 11; MAP kinase p38 beta; MAPK 11; MAPK11; mitogen-activated protein kinase 11; Mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; p38 beta; p38b; p38Beta; P38BETA2; PRKM11; SAPK2B; SAPK2p38-2P38B; Stress-activated protein kinase 2; stress-activated protein kinase-2; stress-activated protein kinase-2b
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