Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

Newer Version Available: DPTX30B
Human Pentraxin 3/TSG-14 Standard Curve
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Citations (19)
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Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (100 uL), EDTA Plasma (100 uL), Saliva (100 uL)
Sensitivity
0.116 ng/mL
Assay Range
0.3 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)
Specificity
Natural and recombinant human PTX3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human PTX3 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human PTX3 in pretreated cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human PTX3 and has been shown to accurately quantitate the recombinant factor. Results obtained using pretreated natural human PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PTX3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum-free Cell Culture Supernates, EDTA Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 2.61 7.72 14.1 2.75 7.94 14.5
Standard Deviation 0.01 0.28 0.62 0.17 0.39 0.6
CV% 3.8 3.7 4.4 6.1 5 4.1

Recovery

The recovery of PTX3 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 83-106
Serum-free Cell Culture Media (n=4) 101 95-108

Linearity

To assess the linearity of the assay, pretreated samples containing and/or spiked with high concentrations of PTX3 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Pentraxin 3/TSG-14 ELISA Linearity

Data Examples

Human Pentraxin 3/TSG-14 Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name:
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs:
5806 (Human); 19288 (Mouse)
Alternate Names:
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 20 µL Pretreated Standard, Control, or Sample
  6.   Add 20 µL of pretreated Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Class II obese and healthy pregnant controls exhibit indistinguishable pro- and anti-inflammatory immune responses to Caesarian section
    Authors: C Graham, M Thorleifso, WP Stefura, DJ Funk, KT HayGlass
    Immun Inflamm Dis, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  2. Investigating the role of pentraxin 3 as a biomarker for bacterial infection in subjects with COPD
    Authors: SJ Thulborn, M Dilpazir, K Haldar, V Mistry, CE Brightling, MR Barer, M Bafadhel
    Int J Chron Obstruct Pulmon Dis, 2017;12(0):1199-1205.
    Species: Human
    Sample Types: Sputum
  3. Diagnostic value of Pentraxin-3 in patients with sepsis and septic shock in accordance with latest sepsis-3 definitions
    Authors: S Hamed, M Behnes, D Pauly, D Lepiorz, M Barre, T Becher, S Lang, I Akin, M Borggrefe, T Bertsch, U Hoffmann
    BMC Infect. Dis., 2017;17(1):554.
    Species: Human
    Sample Types: Plasma
  4. The diagnostic value of serum pentraxin 3 levels in pulmonary contusion
    Authors: Suha Turkmen
    Am J Emerg Med, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells
    Sci Rep, 2016;6(0):37128.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Pseudomonas aeruginosa GroEL stimulates production of PTX3 by activating the NF-?B pathway and simultaneously down-regulating microRNA-9
    Infect. Immun, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Sputum pentraxin 3 as a candidate to assess airway inflammation and remodeling in childhood asthma
    Authors: Min Jung Kim
    Medicine (Baltimore), 2016;95(51):e5677.
    Species: Human
    Sample Types: Sputum
  8. Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide.
    Authors: Borghini A, Manetti M, Nacci F, Bellando-Randone S, Guiducci S, Matucci-Cerinic M, Ibba-Manneschi L, Weber E
    PLoS ONE, 2015;10(6):e0130166.
    Species: Human
    Sample Types: Serum
  9. Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease.
    Authors: Luksha L, Stenvinkel P, Hammarqvist F, Carrero JJ, Davidge ST, Kublickiene K
    PLoS ONE, 2012;7(4):e36056.
    Species: Human
    Sample Types: Serum
  10. Plasma cell-free DNA levels are elevated in acute Puumala hantavirus infection.
    Authors: Outinen TK, Kuparinen T, Jylhava J, Leppanen S, Mustonen J, Makela S, Porsti I, Syrjanen J, Vaheri A, Hurme M
    PLoS ONE, 2012;7(2):e31455.
    Species: Human
    Sample Types: Plasma
  11. Chemerin and adiponectin contribute reciprocally to metabolic syndrome.
    Authors: Chu SH, Lee MK, Ahn KY, Im JA, Park MS, Lee DC, Jeon JY, Lee JW
    PLoS ONE, 2012;7(4):e34710.
    Species: Human
    Sample Types: Plasma
  12. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012;7(4):e34965.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. A multi-analyte assay for the non-invasive detection of bladder cancer.
    Authors: Goodison, Steve, Chang, Myron, Dai, Yunfeng, Urquidi, Virginia, Rosser, Charles
    PLoS ONE, 2012;7(10):e47469.
    Species: Human
    Sample Types: Urine
  14. Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma.
    Authors: Diamandis EP, Goodglick L, Planque C, Thornquist MD
    Clin. Cancer Res., 2011;17(8):2395-2399.
    Species: Human
    Sample Types: Serum
  15. Pentraxin 3 (PTX3) is associated with cardiovascular risk factors: the Health 2000 Survey.
    Authors: Jylhava J, Haarala A, Kahonen M, Lehtimaki T, Jula A, Moilanen L, Kesaniemi YA, Nieminen MS, Hurme M
    Clin. Exp. Immunol., 2011;164(2):211-7.
    Species: Human
    Sample Types: Plasma
  16. Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast.
    Authors: Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R
    Cell, 2011;145(6):926-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. High Plasma Level of Long Pentraxin 3 (PTX3) Is Associated with Fatal Disease in Bacteremic Patients: A Prospective Cohort Study.
    Authors: Huttunen R, Hurme M, Aittoniemi J, Huhtala H, Vuento R, Laine J, Jylhava J, Syrjanen J
    PLoS ONE, 2011;6(3):e17653.
    Species: Human
    Sample Types: Plasma
  18. Is pentraxin 3 involved in obesity-induced decrease in arterial distensibility?
    Authors: Miyaki A, Maeda S, Yoshizawa M
    J. Atheroscler. Thromb., 2010;17(3):278-84.
    Species: Human
    Sample Types: Plasma
  19. Genetic variation in pentraxin (PTX) 3 gene associates with PTX3 production and fertility in women.
    Authors: May L, Kuningas M, van Bodegom D
    Biol. Reprod., 2010;82(2):299-304.
    Species: Human
    Sample Types: Cell Culture Supernates

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